Development of a high resolution Y chromosome microarray for improved male infertility diagnosis

Capsule:
Array-comparative genomic hybridization is a robust higher resolution alternative to multiplex polymerase chain reaction for detection of chromosome Y microdeletions, increasing the diagnostic yield in male infertility.

Authors:
Ryan K.C. Yuen, Ph.D., Anna Merkoulovitch, B.CS., Jeffrey R. MacDonald, B.Sc., Matthew Vlasschaert, B.Sc., Kirk Lo, M.D., Ethan Grober, M.D., Christian R. Marshall, Ph.D., Keith A. Jarvi, M.D., Elena Kolomietz, M.D., Ph.D., Stephen W. Scherer, Ph.D.

Volume 101, Issue 4, Pages 1079-1085.e3

Abstract:

Objective:
To develop a novel clinical test using microarray technology as a high-resolution alternative to current methods for detection of known and novel microdeletions on the Y chromosome.

Design:
Custom Agilent 8x15K array comparative genomic hybridization (aCGH) with 10,162 probes on an average probe spacing of 2.5 kb across the euchromatic region of the Y chromosome.

Setting:
Clinical diagnostic laboratory.

Patient(s):
Men with infertility (n = 104) and controls with proven fertility (n = 148).

Intervention(s):
Microarray genotyping of DNA.

Main Outcome Measure(s):
Gene copy number variation determined by log ratio of probe signal intensity against a DNA reference.

Result(s):
Our aCGH experiments found all known AZF microdeletions as well as additional unbalanced structural alterations. In addition to complete AZF microdeletions, we found that AZFc partial deletions represent a risk factor for male infertility. In total, aCGH-based detection achieved a diagnostic yield of ∼11% and also revealed additional potentially etiologic copy number variations requiring further characterization.

Conclusion(s):
The aCGH approach is a reliable high-resolution alternative to multiplex polymerase chain reaction for the discovery of pathogenic chromosome Y microdeletions in male infertility.

  • Martin Johansson

    Congratulations to a very nice article and microarray. I have a question regarding the information in Figure 1. In the part B of the figure one can see a very short deletion marked on the short arm of the Y chromosome. A similar situation is presented in the C part but this time there are two short deletions marked.
    1. Do you see this event in non-deleted samples as well?
    2. What do you believe is the reason behind these very short p-arm deletions?

    Kind regards
    Martin

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