Peripheral blood stem cell transplants do not result in endometrial stromal engraftment

Capsule:
Peripheral blood stem cell transplants did not result in endometrial engraftment in human and non-human primate models.

Authors:
Erin F. Wolff, M.D., Naoya Uchida, M.D., Ph.D., Robert E. Donahue, V.M.D., Mark. E. Metzger, Matthew M. Hsieh, M.D., Lauren L. Libfraind, B.A., Micah J. Hill, D.O., John F. Tisdale, M.D.

Volume 99, Issue 2, Pages 526-532.e2, February 2013

Abstract:

Objective:
To determine whether peripheral blood stem cell transplant (PBSCT) result in engraftment of donor stem cells in the recipient uterus.

Design:
Prospective clinical and laboratory research.

Setting:
Translational medicine research hospital.

Patient(s)/Animal(s):
Macaque and human bone marrow transplant recipients.

Intervention(s):
Rhesus macaques received autologous transduced immunoselected cytokine-mobilized CD34+ cells after total body irradiation. Vector constructs expressed green fluorescent protein. In the human subjects, prior PBSCT subjects underwent endometrial biopsy and bone marrow aspiration. Macaque and human endometrial and bone marrow cells were isolated and cultured. Fluorescent microscopy, flow cytometry, and polymerase chain reaction (PCR) were used to evaluate for the presence of donor-derived cells.

Main Outcome Measure(s):
Presence of donor cells in recipient endometrium and bone marrow stroma.

Result(s):
The macaque endometrial cells did not exhibit evidence of green fluorescent protein labeling. Human endometrial cells were cultured and the absence of donor blood contamination was verified. The PCR evaluation of the human endometrial cells did not demonstrate evidence of donor short tandem repeats.

Conclusion(s):
The PBSCT did not result in engraftment of donor-derived cells in the endometrium.

  • Micah Hill

    We appreciate the opportunity to present this research in the journal. Prior reports have demonstrated endometrial engraftment from bone marrow mesenchymal cell transplants. In this study of both human and macaque models, we demonstrated no such endometrial engraftment from peripheral blood stem cell transplant. Any questions or discussion would be welcome.

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