Expression of Phosphoinositide-specific Phospholipase C enzymes in normal endometrium and in endometriosis

Capsule:
The different expression of PI-PLC enzymes in the endometrium and endometriosis tissue suggests the hypothesis that PI-PLC enzymes, main effectors in PI signaling, might be involved in the pathogenesis of endometriosis.

Authors:
Vincenza Rita Lo Vasco, M.D., Ph.D., Martina Leopizzi, Ph.D., Caterina Chiappetta, Ph.D., Rita Businaro, M.D., Ph.D., Patrizia Polonia, M.D., Carlo Della Rocca, M.D., Ph.D., Pietro Litta, M.D.

Volume 98, Issue 2 , Pages 410-414, August 2012

Abstract:

Objective:
To delineate the panel of expression of phosphoinositide-specific phospholipase C (PI-PLC) signaling enzymes in normal endometrium and in endometriosis.

Design:
Clinical/experimental study.

Setting:
University.

Patient(s):
Healthy donor woman and endometriosis-affected woman.

Intervention(s):
Normal endometrium and endometriosis surgical biopsies were analyzed using gene expression analyses methodology (reverse transcriptase-polymerase chain reaction [PCR], bioanalyses).

Main Outcome Measure(s):
Gene expression (messenger RNA concentration) measures of 12 PI-PLC enzymes: PI-PLC β1, PI-PLC β2, PI-PLC β3, PI-PLC β4, PI-PLC γ1, PI-PLC γ2, PI-PLC δ1, PI-PLC δ3, PI-PLC δ4, PI-PLC ε, PI-PLC η1, and PI-PLC η2.

Result(s):
PI-PLC β1, PI-PLC β3, PI-PLC δ1, and PI-PLC δ3 enzymes were detected, although differently expressed in normal and endometriosis tissues.

Conclusion(s):
The involvement of PI-PLC enzymes in inflammation and the consistency of susceptible endometriosis loci with PI-PLC genes mapping corroborate the hypothesis that PI signaling might be involved in the pathogenesis of endometriosis.

  • kivan

    Nice study, and very beautiful SEM images. While no difference in pinopode morphology or coverage was identified there seemed to be a trend toward a temporal shift in developmental stage between the 2 groups (table 1). Was this evaluated?

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