Differential expression of G protein coupled estrogen receptor 30 in human myometrial and uterine leiomyoma smooth muscle
G-protein-coupled estrogen receptor-30 (GPR30) is highly expressed in uterine leiomyoma tissue and localized in the nuclei of leiomyoma cells; 17b-estradiol increases GPR30 expression in cultured leiomyoma smooth muscle cells.
Ruijuan Tian, M.Sc., Zengyong Wang, M.Sc., Zhan Shi, M.D., Dong Li, Ph.D., Yuebing Wang, Ph.D., Yingjun Zhu, M.D., Ph.D., Wanjun Lin, M.D., Yu Gui, M.D., Ph.D., Xi-Long Zheng, M.D., Ph.D.
Volume 99, Issue 1, Pages 256-263.e3, January 2013
To determine differential expression of G-protein-coupled receptor 30 (GPR30) in uterine leiomyoma and its matched myometrium.
GPR30 expression was examined in both tissues and cultured cells.
Women 35 to 50 years old with uterine leiomyomas.
Main Outcome Measure(s):
GPR30 expression profile.
Using Western blot and RT-qPCR analyses, we found that GPR30 was highly expressed in uterine leiomyomas compared with their matched myometrium. In only 3 out of 9 patients examined was GPR30 protein detectable by Western blot analysis in myometrial tissues, but at significantly lower levels than in their leiomyomas. Confocal microscopy revealed the nuclear localization of GPR30 in leiomyoma tissues and cultured leiomyoma smooth muscle cells (SMCs). Treatment with 0.1 μM 17-β-estradiol (E2) increased mRNA expression of GPR30 in leiomyoma SMCs, but decreased expression in myometrium SMCs. Treatment with G-1, a GPR30 agonist, stimulated phosphorylation of p44/42 mitogen-activated protein kinase (MAPK) in both SMC types. PD98059, the MEK inhibitor, completely inhibited G-1-induced phosphorylation of p44/42 in myometrium SMCs, but not in SMCs from leiomyoma.
GPR30 is abundantly expressed in uterine leiomyomas, likely resulting from estrogen stimulation.