Interleukin 17A is present in neutrophils in endometrioma and stimulates the secretion of growth related oncogene α Gro α from endometrioma stromal cells

Capsule:
Interleukin (IL)-17A was present in neutrophils in endometrioma and it increased Gro-a secretion from endometrioma stromal cells, which induced neutrophil migration. IL-17A in neutrophils may contribute to the development of endometriosis.

Authors:
Masashi Takamura, M.D., Ph.D., Yutaka Osuga, M.D., Ph.D., Gentaro Izumi, M.D., Osamu Yoshino, M.D., Ph.D., Kaori Koga, M.D., Ph.D., Ako Saito, M.D., Ph.D., Tetsuya Hirata, M.D., Ph.D., Yasushi Hirota, M.D., Ph.D., Miyuki Harada, M.D., Ph.D., Akiko Hasegawa, M.D., Ph.D., Yuji Taketani , M.D., Ph.D.

Volume 98, Issue 5, Pages 1218-1224.e2, November 2012

Abstract:

Objective:
To investigate a new role of IL-17A in endometriosis.

Design:
Laboratory study.

Setting:
University Hospital.

Patients:
Patients with ovarian endometrioma undergoing laparoscopy or laparotomy.

Interventions:
Primary culture of endometrioma stromal cells (EoSCs) was stimulated with IL-17A. Sections of endometrioma tissue were immunostained with antibodies for IL-17A, Gro-α, and elastase, a marker of neutrophils. They were also examined with immunofluorescent double staining for IL-17A and myeloperoxidase, another marker of neutrophils.

Main Outcome Measure:
Concentration of Gro-α was measured using a specific ELISA. Neutrophil chemotaxis was measured with Boyden chamber method. Immunostained sections were examined under microscope.

Results:
Interleukin-17A increased the secretion of Gro-α from EoSCs dose-dependently. The conditioned medium of EoSCs stimulated with IL-17A attracted more neutrophils than that of EoSCs stimulated with vehicle, and the increase was inhibited by the addition of Gro-α-neutralizing antibody. On immunostaining, IL-17A and Gro-α were detected in similar areas of the stroma beneath the epithelium, where Gro-α was detected in cells with a stromal cell appearance whereas IL-17A was detected in neutrophils as determined by detection of elastase. Fluorescent immunostaining corroborated that myeloperoxidase-positive neutrophils were also positive for IL-17A.

Conclusion(s):
It is suggested that IL-17A produced by neutrophils stimulates Gro-α secretion from EoSCs, thereby recruiting more neutrophils and inducing perpetuating inflammation in endometriosis.

  • Juan Garcia-Velasco, MD

    Congratulations for your reseach! This is a beautiful piece of work that helps to improve the understanding the complex puzzle of this disease and its pathogenesis. Do you consider, even speculate, that IL-antagonist might have a future in clinical trials?

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