Factors associated with birth outcomes from cryopreserved blastocysts Experience from 4597 autologous transfers of 7597 cryopreserved blastocysts

Wednesday, April 13, 2016

Authors:
Kevin S. Richter, Ph.D., Daniella K.…

Read More

Six years experience in ovum donation using vitrified oocytes Report of cumulative outcomes impact of storage time and development of a predictive model for oocyte survival rate

Monday, November 30, 2015
Oocyte banking is clinically efficient. Estimation of the likelihood of having a child is provided. There is no way of estimating donors’ oocytes survival when considering baseline characteristics, storage time, or controlled ovarian stimulation parameters.

Read More

Freeze all at the blastocyst or bipronuclear stage A randomized clinical trial

Thursday, October 29, 2015
Outcomes using embryos cryopreserved at the blastocyst stage or bipronuclear stage followed by thaw and culture to the blastocyst stage did not differ significantly.

Read More

Single vitrified blastocyst transfer maximizes liveborn children per embryo while minimizing preterm birth

Monday, June 1, 2015
Single vitrified-warmed blastocyst transfer provides excellent live-birth rates and higher embryo to live-birth efficiency while minimizing preterm birth and low birth weight, relative to double transfer.

Read More

Baby budgeting Oocyte cryopreservation in women delaying reproduction can reduce cost per live birth

Monday, June 1, 2015
This cost analysis demonstrates that in women who plan to delay childbearing until age 40 years, oocyte cryopreservation before 38 years of age can reduce the cost to obtain a live birth.

Read More

Blastocysts can be rebiopsied for preimplantation genetic diagnosis and screening

Tuesday, November 25, 2014
We found that blastocysts can survive two rounds of biopsy, vitrification, and warming with a high implantation rate. Blastocyst re-examination may be clinically valuable in preimplantation genetic diagnosis/screening cycles.

Read More

Cryopreservation of human embryos and its contribution to in vitro fertilization success rates

Wednesday, June 25, 2014
The success rates after frozen-thawed embryo transfer are nearing those of fresh transfer in assisted reproductive technology cycles. Randomized controlled trials should determine whether fresh transfers should be abandoned.

Read More

Effect of oocytes vitrification on deoxyribonucleic acid methylation of H19 Peg3 and Snrpn differentially methylated regions in mouse blastocysts

Monday, June 23, 2014
Vitrification can lead to a loss of deoxyribonucleic acid methylation of imprinted genes in blastocysts, possibly because of reduction of deoxyribonucleic acid methyltransferase abundance in mouse MII oocytes after vitrification.

Read More

Single embryo transfer of vitrified warmed blastocysts yields equivalent live birth rates and improved neonatal outcomes compared with fresh transfers

Wednesday, April 30, 2014
After 1,157 fresh and 645 vitrified-warmed day-5 single-blastocyst transfers, we found vitrified warmed blastocysts yielded equivalent birth rates and improved neonatal outcomes compared with fresh transfers.

Read More

Improving ovarian tissue cryopreservation for oncologic patients Slow freezing versus vitrification effect of different procedures and devices

Thursday, February 20, 2014
Vitrification of ovarian tissue employing ethylene glycol and dimethyl sulfoxide in metallic grids preserves morphology of follicles and stroma, and maintains proliferation, viability, and vascularization status similarly to fresh tissue.

Read More

Comprehensive chromosome screening of trophectoderm with vitrification facilitates elective single embryo transfer for infertile women with advanced maternal age

Friday, August 30, 2013
Comprehensive chromosome screening of trophectoderm with vitrification and later transfer in an unstimulated cycle may be the key to providing elective single-embryo transfer for the older woman undergoing IVF.

Read More

Age specific probability of live birth with oocyte cryopreservation An individual patient data meta analysis

Wednesday, July 31, 2013
Age-specific probabilities of live birth calculated in this report would enable more accurate counseling and informed decisions for infertile women considering oocyte cryopreservation.

Read More

Preservation of human ovarian follicles within tissue frozen by vitrification in a xeno free closed system using only ethylene glycol as a permeating cryoprotectant

Friday, June 28, 2013
Clinical-grade vitrification of human ovarian tissue in fertility preservation, with the use of only one permeating cryoprotectant, ethylene glycol, in a closed system results in intact follicular ultrastructure.

Read More

Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o histone acetyltransferase 1 and deacetylase 1 in metaphase II mouse oocytes

Friday, June 28, 2013
Vitrification does not significantly affect the methylation patterns of the CpG islands in the Dnmt1o, Hat1, and Hdac1 promoters in mouse metaphase II oocytes, but reduces expression of Dnmt1o mRNA.

Read More

In vitro maturation of human immature oocytes for fertility preservation

Thursday, March 28, 2013
Pregnancies achieved after cryopreservation of in vitro and in vivo matured oocytes are not associated with adverse pregnancy outcomes and may represent a novel strategy of fertility preservation.

Read More

The impact of next generation sequencing technology on preimplantation genetic diagnosis and screening

Monday, March 18, 2013
Emergence of new IVF techniques and massively parallel DNA sequencing provide deep comprehensive genome-wide sequence readout as an end point to screen for and diagnose chromosomal abnormalities and single-gene disorders.

Read More

Effect of vitrification on human oocytes A metabolic profiling study

Monday, February 4, 2013
Global metabolomic and amino acid profiles of spent culture media samples from embryos derived from either fresh or vitrified oocytes showed no statistically significant differences.

Read More

Mature oocyte cryopreservation: a guideline

Wednesday, January 2, 2013
Evidence indicates that vitrification and warming of unfertilized oocytes followed by fertilization, commonly by intracytoplasmic sperm injection (ICSI), result in subsequent pregnancy rates which are acceptable and this technique should no longer be considered experimental.

Read More

Allotransplantation of cryopreserved prepubertal mouse ovaries restored puberty and fertility without affection methylation profile of Snrpn DMR

Wednesday, January 2, 2013
Vitrification of prepubertal mouse ovaries did not affect the methylation profile of Snrpn-DMR or their development and fertility potential.

Read More

Vitrification and xenografting of human ovarian tissue

Thursday, November 1, 2012
Two vitrification protocols were shown to preserve the morphology and viability of human preantral follicles. After warming and xenografting to nude mice for 1 week, these follicles were able to survive and develop.

Read More

A reliable procedure for decontamination before thawing of human specimens cryostored in liquid nitrogen Three washes with sterile liquid nitrogen SLN2

Thursday, September 27, 2012
Three subsequent washings in certified ultraviolet sterile liquid nitrogen produce an efficient decontamination of vitrification carriers, allowing safe thawing of human specimens.

Read More

Epigenetics and vitrification of embryos: one step forward and many raised equivocal questions

Thursday, June 28, 2012
The author reflects on the findings of Zhao et al. in their article "Effect of 5-aza-2′-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos" and discusses the need for additional research and experience in epigenetics before the new technology can be applied to human IVF and ART.

Read More

Effect of 5-aza-2′-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos

Thursday, June 7, 2012
H19 imprinted control region methylation levels significantly increased in vitrified two-cell embryos and their derived blastocysts; 5-aza-dC significantly reduced H19 imprinted control region methylation and increased H19 expression in blastocysts derived from vitrified embryos.

Read More

Successful pregnancy in vitrified/warmed blastocyst intrafallopian transfer

Wednesday, June 6, 2012
Blastocyst intrafallopian transfer is a feasible option in cases of repeated difficult ETs, regardless of whether the patient shows cervical adhesions or any type of genital malformations.

Read More

Comparison of Cryotip vs. Cryotop for mouse and human blastomere vitrification

Monday, June 4, 2012
We have compared open vs. closed systems for mouse and human blastomere vitrification and conclude that the closed system is more efficient in terms of blastomere survival and division.

Read More

Perinatal and neonatal outcomes of 494 babies delivered from 972 vitrified embryo transfers

Wednesday, May 30, 2012
Compared with fresh embryo transfers, vitrified day 3 embryo transfer shows no significant differences in obstetrical and neonatal outcomes. Vitrification is a safe method for the cryopreservation of human day 3 embryos.

Read More

Orthotopic grafting of cryopreserved prepubertal testicular tissue: in search of a simple yet effective cryopreservation protocol

Friday, May 4, 2012
Solid-surface vitrification resulted in success rates similar to conventional uncontrolled slow freezing inpreserving the integrity and function of prepubertalmouse testicular tissue.

Read More

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Friday, May 4, 2012
Initial findings suggest that a moderate concentration of the cryoprotectant dimethyl sulfoxide (DMSO) is superior to a high DMSO concentration for both vitrification and slow freezing.

Read More

Efficiency of slush nitrogen vitrification of human oocytes vitrified with or without cumulus cells in relation to survival rate and meiotic spindle competence

Friday, May 4, 2012
Complete denudation of oocytes before slush nitrogen vitrification does not affect survival rate but is associated with a higher incidence of postthaw meiotic spindle visualization.

Read More

Translate »