Relationship between sperm aneuploidy sperm DNA integrity chromatin packaging traditional semen parameters and recurrent pregnancy loss

Tuesday, December 29, 2015
The increase in abnormal sperm parameters, sperm DNA fragmentation, chromatin decondensation, and aneuploidy suggest possible causes of unexplained recurrent pregnancy loss, which encourage the screening of both partners simultaneously.

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Role of spermatozoa in the etiology of miscarriage

Tuesday, December 29, 2015

Author:
Richard Bronson, M.D.…

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Increased chromosome 16 disomy rates in human spermatozoa and recurrent spontaneous abortions

Thursday, October 29, 2015
Meiotic errors involving chromosome 16 of paternal origin seem to have an equally important influence on to the overall fetal aneuploidy rate compared with maternal chromosome 16 disomy.

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Spermatozoa from patients with seminal alterations exhibit a differential micro ribonucleic acid profile

Tuesday, September 1, 2015
Spermatozoa from infertile patients with asthenozoospermia, teratozoospermia, or oligozoospermia exhibit a differential miRNA profile associated with biological processes related to their particular seminal alterations.

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Concordance among sperm deoxyribonucleic acid integrity assays and semen parameters

Monday, June 29, 2015
The sperm chromatin structure assay and TUNEL assay are moderately correlated measures of sperm DNA integrity but often yield conflicting results. The DNA fragmentation index is well correlated with semen analysis parameters, whereas TUNEL is not.

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Histone methylation and acetylation in ejaculated human sperm Effects of swim up and smoking

Monday, June 1, 2015
This study demonstrates a decrease of global methylation and unchanged acetylation level in ejaculated human sperm after the swim-up preparation.

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Proteomic identification of target proteins in normal but nonfertilizing sperm

Wednesday, July 30, 2014
The objective of this study was to identify male molecular causes of fertilization failures. Samples were analyzed by proteomic analysis. Two proteins involved in gametic interaction were identified: LR67 receptor and xylulose reductase.

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Panel of five microRNAs as potential biomarkers for the diagnosis and assessment of male infertility

Tuesday, July 1, 2014
Quantitative real-time polymerase chain reaction validation reveals that a set of five miRNAs deserves to be used as a diagnostic test along with other routine clinical tests, such as conventional semen and histologic analysis, to improve male infertility assessment and diagnosis.

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Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation

Tuesday, May 27, 2014
A single day of ejaculatory abstinence (EA) compared with 4 days of EA increased seminal total antioxidant capacity without a measurable effect on sperm membrane lipid peroxidation.

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Dendritic cells in semen of infertile men Association with sperm quality and inflammatory status of the epididymis

Tuesday, December 31, 2013
The inflammatory status of the epididymis may be reflected by the abundance and activation status of dendritic cells in semen, which may affect sperm quality and fertility outcome.

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Paternal age and sperm methylation status

Monday, September 30, 2013
Reflections on "Paternal aging and associated intraindividual alterations of global sperm 5-methylcytosine and 5-hydroxymethylcytosine levels" by Jenkins et al.

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Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting

Friday, August 30, 2013
By combining the staining of apoptotic and dead spermatozoa with YO-PRO 1 and cytometric cell sorting, we obtained highly enriched populations of viable spermatozoa with reduced DNA fragmentation.

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Filling the void about sperm function knowledge and how the omics approach can close the circle

Wednesday, July 31, 2013
Reflections on "Proteomic analysis of seminal plasma from infertile patients with oligoasthenoteratozoospermia due to oxidative stress and comparison with fertile volunteers" by Herwig et al.

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Identification of a novel sperm motility stimulating protein from caprine serum its characterization and functional significance

Friday, June 28, 2013
A sperm forward motility–stimulating protein was purified from goat serum and characterized. The research provides a potential nonsteroidal biomolecule and its antibody that may solve some global reproductive problems.

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In vitro fertilization intracytoplasmic sperm injection outcome in patients with a markedly high DNA fragmentation index greater than 50 percent

Friday, June 28, 2013
Similar fertilization and pregnancy rates were found in ICSI cycles involving men with markedly high DNA damage (DNA fragmentation index >50) compared with healthy men and controlled for partner variables.

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Two dimensional differential in gel electrophoresis based proteomics of male gametes in relation to oxidative stress

Thursday, March 28, 2013
Proteomic analysis shows that differences in protein expression in spermatozoa producing higher levels of ROS may play a role in the pathophysiology of male infertility.

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Proteomic analysis and sperm physiopathology The two dimensional difference in gel electrophoresis approach

Thursday, March 28, 2013
Reflections on Hamada et al. "Two-Dimensional Differential In-Gel Electrophoresis-based proteomics of male gametes in relation to oxidative stress"

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Altered microRNA expression profiles of human spermatozoa in patients with different spermatogenic impairments

Thursday, March 28, 2013
Microarray and real-time polymerase chain reaction (PCR) studies reveal that microRNAs are differentially expressed in normal versus spermatogenically impaired males and have therapeutic potential for the development of biomarkers for male infertility.

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Assessment of sperm using mRNA microarray technology

Monday, March 18, 2013
Sperm mRNA microarray analysis is a promising methodology currently under development, which allows sperm molecular quality to be determined and complements basic sperm analysis diagnoses. In addition, it may help to design new sperm selection tools.

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Involvement of sperm plasma membrane and cytoskeletal proteins in human male infertility

Friday, March 1, 2013
Asthenozoospermic spermatozoa exhibited a modification in plasma membrane rheology, and a reduced immunohistochemical expression of ezrin, Cdc42, CD9, F-actin, and b-tubulin, which could contribute to sperm functional impairment.

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Human sperm head vacuoles are physiological structures formed during the sperm development and maturation process

Friday, June 8, 2012
Human sperm head vacuoles have no effect on intracytoplasmic sperm injection outcomes because they are not abnormal structures but physiological changes occurring during the sperm development and maturation process.

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Development and current applications of assisted fertilization

Thursday, May 24, 2012
We describe the early steps that brought forth the development of intracytoplasmic sperm injection and its role in assisted reproduction techniques.

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