Use of testicular sperm for intracytoplasmic sperm injection in oligozoospermic couples with high levels of sperm deoxyribonucleic acid fragmentation

Monday, November 30, 2015

Author:
Armand Zini, M.D.

Volume 104, Issue 6, Pages 1376-1377

Abstract:

Reflections on “Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm” by Esteves et al.…

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Analysis of sperm membrane charge using microelectrophoresis demonstrates that semen samples with a higher proportion of negatively charged sperm are associated with improved ART outcomes.

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Human semen air-dried before DNA fragmentation analysis provides statistically significantly closer results to fresh semen compared with snap-frozen semen and can be stored for up to 1 month.

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Data from this study suggest that seminal hyperviscosity (a posttesticular factor) is not an important cause of sperm DNA damage and is not associated with semenoglin degradation.

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Tuesday, December 31, 2013
Sperm chromatin dispersion (SCD) and TUNEL are poorly correlated for estimating DNA damage in men with unexplained infertility. SCD discriminates normal and abnormal sperm DNA damage with up to 69% accuracy when compared with TUNEL.

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Commentary on "Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting" by Ribeiro et al.

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Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in PVP-based sperm immobilization medium

Friday, June 8, 2012
Polyvinylpyrrolidone-based medium can induce sperm DNA fragmentation during intracytoplasmic sperm injection, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

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