Use of testicular sperm for intracytoplasmic sperm injection in oligozoospermic couples with high levels of sperm deoxyribonucleic acid fragmentation

Monday, November 30, 2015

Armand Zini, M.D.

Volume 104, Issue 6, Pages 1376-1377


Reflections on “Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated and testicular sperm” by Esteves et al.…

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Microelectrophoresis A noninvasive method of sperm selection based on membrane charge

Friday, January 30, 2015
Analysis of sperm membrane charge using microelectrophoresis demonstrates that semen samples with a higher proportion of negatively charged sperm are associated with improved ART outcomes.

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Development of a simplified method of human semen storage for the testing of sperm DNA fragmentation using the Halosperm G2 test kit

Wednesday, July 9, 2014
Human semen air-dried before DNA fragmentation analysis provides statistically significantly closer results to fresh semen compared with snap-frozen semen and can be stored for up to 1 month.

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Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization intracytoplasmic sperm injection A systematic review and metaanalysis

Friday, June 20, 2014
This systematic review and meta-analysis showed that high sperm DNA fragmentation was related to lower pregnancy rates in IVF but not in ICSI cycles, whereas it was associated to higher miscarriage rates in both IVF and ICSI cycles.

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Seminal hyperviscosity is not associated with semenogelin degradation or sperm deoxyribonucleic acid damage A prospective study of infertile couples

Sunday, June 1, 2014
Data from this study suggest that seminal hyperviscosity (a posttesticular factor) is not an important cause of sperm DNA damage and is not associated with semenoglin degradation.

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Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility

Tuesday, December 31, 2013
Sperm chromatin dispersion (SCD) and TUNEL are poorly correlated for estimating DNA damage in men with unexplained infertility. SCD discriminates normal and abnormal sperm DNA damage with up to 69% accuracy when compared with TUNEL.

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Sperm sorting for selection of healthy sperm is it safe and useful

Friday, August 30, 2013
Commentary on "Isolation of spermatozoa with low levels of fragmented DNA with the use of flow cytometry and sorting" by Ribeiro et al.

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Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in PVP-based sperm immobilization medium

Friday, June 8, 2012
Polyvinylpyrrolidone-based medium can induce sperm DNA fragmentation during intracytoplasmic sperm injection, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

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