Monday, June 29, 2015
Leukocytospermia (1 106 leukocytes/mL), assessed by flow cytometry, does not significantly influence IVF or ICSI outcomes. The same results were obtained by using lower or higher cut-off values for leukocytospermia (from 0.2 to 2 106/mL).
Friday, May 31, 2013
This study establishes a simple method for in vitro sperm head decondensation, which allows an accurate detection of real sperm DNA content and direct quantification of diploid sperm.
Monday, June 4, 2012
Immature testicular tissue is more sensitive and shows differential cell-specificity to cryoprotectants. Dimethyl sulfoxide and ethylene glycol are ideal cryoprotectants for immature and adult testicular tissue, respectively.
Wednesday, May 30, 2012
Fluorescent Pisum sativum agglutinin rapidly labels and stabilizes the acrosomal matrix when exocytic fusion pores open in live human sperm, as assessed by confocal microscopy, electron microscopy, and flow cytometry.