Effect of seminal leukocytes on in vitro fertilization and intracytoplasmic sperm injection outcomes

Monday, June 29, 2015
Leukocytospermia (1  106 leukocytes/mL), assessed by flow cytometry, does not significantly influence IVF or ICSI outcomes. The same results were obtained by using lower or higher cut-off values for leukocytospermia (from 0.2 to 2  106/mL).

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A novel in vitro sperm head decondensation protocol for rapid flow cytometric measurement of deoxyribonucleic acid content

Friday, May 31, 2013
This study establishes a simple method for in vitro sperm head decondensation, which allows an accurate detection of real sperm DNA content and direct quantification of diploid sperm.

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Efficient cryopreservation of testicular tissue: effect of age, sample state, and concentration of cryoprotectant

Monday, June 4, 2012
Immature testicular tissue is more sensitive and shows differential cell-specificity to cryoprotectants. Dimethyl sulfoxide and ethylene glycol are ideal cryoprotectants for immature and adult testicular tissue, respectively.

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Recording and sorting live human sperm undergoing acrosome reaction

Wednesday, May 30, 2012
Fluorescent Pisum sativum agglutinin rapidly labels and stabilizes the acrosomal matrix when exocytic fusion pores open in live human sperm, as assessed by confocal microscopy, electron microscopy, and flow cytometry.

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