Embryology in the era of proteomics
Monday, March 18, 2013
A review of the contribution of proteomic technologies in the identification of the human embryonic secretome.
A review of the contribution of proteomic technologies in the identification of the human embryonic secretome.
Blastocyst embryo transfer improves the live-birth rate in good prognosis patients compared with cleavage-stage transfer. In unselected or poor prognosis patients, it is not proven superior to cleavage-stage transfer.
H19 imprinted control region methylation levels significantly increased in vitrified two-cell embryos and their derived blastocysts; 5-aza-dC significantly reduced H19 imprinted control region methylation and increased H19 expression in blastocysts derived from vitrified embryos.
We have compared open vs. closed systems for mouse and human blastomere vitrification and conclude that the closed system is more efficient in terms of blastomere survival and division.
Technologies related to automation, such as nonsubjective sperm selection, oocyte fertilization, and embryo culture and monitoring, are available, and this means that sample preparation, chemical or biologic reactions, and data collection could be integrated.
Recurrent implantation failure in IVF may be partially attributed to oocyte, sperm, and embryonic factors. Possible etiologies are reviewed and discussed, and recent advances in oocyte and embryo selection are evaluated along with current recommended management strategies.