The delivery rate after vitrification of embryos derived from vitrified oocytes is not impaired by double vitrification.
A review of 60 cases of frozen-thawed ovarian tissue reimplantation is presented. Cryopreservation of ovarian tissue is the only option available for prepubertal girls and for women who cannot delay the start of chemotherapy.
Human preantral follicles can be successfully cryopreserved before or after isolation without impairing their ability to survive and grow in vitro.
Dimethylsulfoxide may up-regulate AQP7 expression in mouse oocytes by both osmolarity-dependent and osmolarity-independent pathways, and up-regulation of AQP7 facilitates water diffusion in mouse oocytes.
Reflections on “Elective cryopreservation of all embryos with subsequent cryothaw embryo transfer in patients at risk for ovarian hyperstimulation syndrome reduces the risk of adverse obstetric outcomes: a preliminary study” by Imudia et al.
The delivery rate achieved after the vitrification of early cleavage- and blastocyst-stage embryos is not affected by the embryo developmental stage or any other variable related to the warming cycle.
Two vitrification protocols were shown to preserve the morphology and viability of human preantral follicles. After warming and xenografting to nude mice for 1 week, these follicles were able to survive and develop.
Ovarian function was restored and pregnancy achieved in a woman who had previously undergone bilateral oophorectomy for benign disease and in whom frozen-thawed ovarian cortex was subsequently autotransplanted.
All IVF programs and clinics should have plans for protection of fresh and cryopreserved human tissue and continuation of patient care in the event of an emergency or natural disaster.
Immature testicular tissue is more sensitive and shows differential cell-specificity to cryoprotectants. Dimethyl sulfoxide and ethylene glycol are ideal cryoprotectants for immature and adult testicular tissue, respectively.
The follicle pool in transplanted frozen/thawed ovarian tissue is low compared with normal ovaries, and reduced follicular selection may explain these women’s poor reproductive outcomes in assisted reproduction.
This review summarizes state-of-the-art of ovarian transplantation and cryopreservation, and the limits and future prospects of these new techniques.
High intra-age, intercycle, and intercenter variation in oocyte aneuploidy was observed in first polar body chromosome analysis of oocyctes.
We report on how having embryos frozen is associated with good quality embryos but not having embryos frozen is not a marker of poor embryo quality.
Solid-surface vitrification resulted in success rates similar to conventional uncontrolled slow freezing inpreserving the integrity and function of prepubertalmouse testicular tissue.
Initial findings suggest that a moderate concentration of the cryoprotectant dimethyl sulfoxide (DMSO) is superior to a high DMSO concentration for both vitrification and slow freezing.
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