Recommendations for development of an emergency plan for in vitro fertilization programs A committee opinion

Sunday, May 1, 2016
All in vitro fertilization programs and clinics should have plans for the protection of fresh and cryopreserved human tissue and continuation of patient care in the event of an emergency or natural disaster.

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Factors associated with birth outcomes from cryopreserved blastocysts Experience from 4597 autologous transfers of 7597 cryopreserved blastocysts

Wednesday, April 13, 2016

Authors:
Kevin S. Richter, Ph.D., Daniella K.…

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Cryopreservation and recovery of human endometrial epithelial cells with high viability purity and functional fidelity

Monday, February 1, 2016
A protocol for cryopreservation of human endometrial epithelial cells allows for successful recovery and establishment of functional characteristics.

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Female fertility preservation Innovations and questions

Tuesday, December 29, 2015
Preserving the potential of becoming genetic mother should now be part of the management of infants, adolescents, and young women at risk for infertility from benign or malignant diseases.

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Cryopreservation of testicular tissue before long term testicular cell culture does not alter in vitro cell dynamics

Thursday, October 29, 2015
Cryopreservation of testicular tissue before culture does not influence the cell composition in longterm testicular cell cultures.

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Freezing and thawing testicular cells may not affect growth of cultured testicular cells

Thursday, October 29, 2015

Authors:
James F. Smith, M.D., M.S., Nam D.…

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Single human sperm cryopreservation method using hollow core agarose capsules

Tuesday, September 29, 2015
A cryopreservation method for a few spermatozoa using an agarose capsule was established. The method will be useful for intracytoplasmic sperm injection treatment in patients with few spermatozoa.

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Antimullerian hormone as a predictor of good-quality supernumerary blastocyst cryopreservation among women with levels <1 ng mL versus 1 4 ng mL

Tuesday, September 1, 2015
Antimullerian hormone (AMH) levels <1 ng/mL are associated with a statistically significantly lower likelihood of supernumerary good-quality blastocyst cryopreservation as compared with AMH levels between 1 and 4 ng/mL.

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Liability for mismanagement of sperm specimens in fertility practices

Monday, December 29, 2014

Authors:
Micah Vaughn, B.S., Amjad Hossain, Ph.D., John Y.…

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Upregulation of heme oxygenase 1 expression modulates reactive oxygen species level during the cryopreservation of human seminiferous tubules

Wednesday, July 9, 2014
The ROS declined and HO-1 increased after the thawing of human seminiferous tubules. Slow freezing is more effective than vitrification in terms of HO-1 up-regulation and ROS decrease.

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Effect of sugar molecules on the cryopreservation of mouse spermatogonial stem cells

Monday, March 31, 2014
Spermatogonial stem cells cryopreserved in sugar molecules have greater post-thaw survival compared with stem cells preserved in standard freezing medium.

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Is ex vivo microdissection testicular sperm extraction indicated for infertile men undergoing radical orchiectomy for testicular cancer Case report and literature review

Monday, March 31, 2014
Areas of spermatogenesis may be missed with conventional testicular sperm extraction (TESE) in azoospermic men with testicular cancer. Microdissection TESE should be offered at orchiectomy to maximize sperm retrieval.

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Impact of fresh versus cryopreserved testicular sperm upon intracytoplasmic sperm injection pregnancy outcomes in men with azoospermia due to spermatogenic dysfunction a meta analysis

Friday, January 31, 2014
After review and meta-analysis of the use of fresh versus cryopreserved sperm for intracytoplasmic sperm injection in azoospermic men with spermatogenic dysfunction, we found no difference in pregnancy or fertilization rates.

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Fertility preservation and reproduction in patients facing gonadotoxic therapies a committee opinion

Thursday, October 31, 2013
Ethical considerations of preserving fertility after gonadotoxic treatment are discussed.

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Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries

Monday, September 30, 2013
Whole-ovary cryopreservation is inefficient because of the large organ volume. A direct comparison between conventional slow freezing and directional freezing reveals that the latter leaves whole organs largely intact.

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Disposition of abandoned embryos A committee opinion

Friday, May 31, 2013
It is ethically acceptable to destroy abandoned embryos. It is not ethically acceptable to use abandoned embryos for research or transfer than to another couple without prior written consent.

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Prospective controlled study to evaluate laboratory and clinical outcomes of oocyte vitrification obtained in in vitro fertilization patients aged 30 to 39 years

Friday, May 31, 2013
In a sibling oocyte study conducted in infertile patients 30–39 years of age, oocyte vitrification had minimal negative impact on embryo development and subsequent clinical results.

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Outcome of cryotransfer of embryos developed from vitrified oocytes double vitrification has no impact on delivery rates

Tuesday, April 30, 2013
The delivery rate after vitrification of embryos derived from vitrified oocytes is not impaired by double vitrification.

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Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue a review of 60 cases of reimplantation

Tuesday, April 30, 2013
A review of 60 cases of frozen-thawed ovarian tissue reimplantation is presented. Cryopreservation of ovarian tissue is the only option available for prepubertal girls and for women who cannot delay the start of chemotherapy.

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Should we isolate human preantral follicles before or after cryopreservation of ovarian tissue

Thursday, March 28, 2013
Human preantral follicles can be successfully cryopreserved before or after isolation without impairing their ability to survive and grow in vitro.

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Cryoprotectants up regulate the expression of mouse oocyte AQP7 which facilitates water diffusion during cryopreservation

Thursday, March 28, 2013
Dimethylsulfoxide may up-regulate AQP7 expression in mouse oocytes by both osmolarity-dependent and osmolarity-independent pathways, and up-regulation of AQP7 facilitates water diffusion in mouse oocytes.

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Modifiable Aspects of IVF to Reduce Perinatal Morbidity

Wednesday, January 2, 2013
Reflections on "Elective cryopreservation of all embryos with subsequent cryothaw embryo transfer in patients at risk for ovarian hyperstimulation syndrome reduces the risk of adverse obstetric outcomes: a preliminary study" by Imudia et al.

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Outcomes of vitrified early cleavage stage and blastocyst stage embryos in a cryopreservation program Evaluation of 3150 warming cycles

Thursday, November 1, 2012
The delivery rate achieved after the vitrification of early cleavage- and blastocyst-stage embryos is not affected by the embryo developmental stage or any other variable related to the warming cycle.

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Vitrification and xenografting of human ovarian tissue

Thursday, November 1, 2012
Two vitrification protocols were shown to preserve the morphology and viability of human preantral follicles. After warming and xenografting to nude mice for 1 week, these follicles were able to survive and develop.

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Live birth after transplantation of frozen-thawed ovarian tissue after bilateral oophorectomy for benign disease

Thursday, June 7, 2012
Ovarian function was restored and pregnancy achieved in a woman who had previously undergone bilateral oophorectomy for benign disease and in whom frozen-thawed ovarian cortex was subsequently autotransplanted.

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Recommendations for development of an emergency plan for in vitro fertilization programs: a committee opinion

Wednesday, June 6, 2012
All IVF programs and clinics should have plans for protection of fresh and cryopreserved human tissue and continuation of patient care in the event of an emergency or natural disaster.

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Efficient cryopreservation of testicular tissue: effect of age, sample state, and concentration of cryoprotectant

Monday, June 4, 2012
Immature testicular tissue is more sensitive and shows differential cell-specificity to cryoprotectants. Dimethyl sulfoxide and ethylene glycol are ideal cryoprotectants for immature and adult testicular tissue, respectively.

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Evaluation of the ovarian reserve in women transplanted with frozen and thawed ovarian cortical tissue

Thursday, May 31, 2012
The follicle pool in transplanted frozen/thawed ovarian tissue is low compared with normal ovaries, and reduced follicular selection may explain these women’s poor reproductive outcomes in assisted reproduction.

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Ovarian tissue and follicle transplantation as an option for fertility preservation

Wednesday, May 30, 2012
This review summarizes state-of-the-art of ovarian transplantation and cryopreservation, and the limits and future prospects of these new techniques.

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Intra-age, intercenter, and intercycle differences in chromosome abnormalities in oocytes

Friday, May 11, 2012
High intra-age, intercycle, and intercenter variation in oocyte aneuploidy was observed in first polar body chromosome analysis of oocyctes.

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