Validation of a next generation sequencing based protocol for 24 chromosome aneuploidy screening of blastocysts

Wednesday, June 1, 2016
Our study validated multiple annealing and looping-based amplification cycle next-generation sequencing for 24-chromosome aneuploidy screening of blastocysts.

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Factors associated with birth outcomes from cryopreserved blastocysts Experience from 4597 autologous transfers of 7597 cryopreserved blastocysts

Wednesday, April 13, 2016

Authors:
Kevin S. Richter, Ph.D., Daniella K.…

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Distribution patterns of segmental aneuploidies in human blastocysts identified by next generation sequencing

Friday, April 1, 2016
Next-generation sequencing allows for the detection of pure and mosaic segmental aneuploidies with the same efficiency as array comparative genomic hybridization.

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Examining the many potential reasons why euploid blastocysts do not always result in viable pregnancies Part 1

Tuesday, March 1, 2016
The environment surrounding the oocyte and embryo from months before ovarian stimulation to embryo transfer profoundly affects the proportion of euploid embryos that fail to result in viable pregnancies.

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Preimplantation genetic testing Polar bodies blastomeres trophectoderm cells or blastocoelic fluid

Tuesday, March 1, 2016
The chromosome condition of DNA retrieved by blastocentesis was highly concordant with that found in trophectoderm cells, polar bodies, and blastomeres. Segmental abnormalities could also be detected in blastocoelic fluids.

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Does the addition of time lapse morphokinetics in the selection of embryos for transfer improve pregnancy rates A randomized controlled trial

Monday, February 1, 2016
This randomized controlled trial could not detect a statistically significant difference in reproductive outcomes when continuous time-lapse morphokinetic data was added to conventional screening criteria for embryo selection.

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Noninferiority randomized controlled trial comparing embryo development using media developed for sequential or undisturbed culture in a time lapse setup

Monday, November 30, 2015
In this randomized sibling study we found no differences in blastocyst development between sequential and single-step media.

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Risk of preterm birth after blastocyst embryo transfer A large population study using contemporary registry data from Australia and New Zealand

Tuesday, September 29, 2015
A large assisted reproductive technology registry study finds that blastocyst transfer does not increase the risk of preterm birth, low birth weight, or small for gestational age.

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Antimullerian hormone as a predictor of good-quality supernumerary blastocyst cryopreservation among women with levels <1 ng mL versus 1 4 ng mL

Tuesday, September 1, 2015
Antimullerian hormone (AMH) levels <1 ng/mL are associated with a statistically significantly lower likelihood of supernumerary good-quality blastocyst cryopreservation as compared with AMH levels between 1 and 4 ng/mL.

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New strategy for diagnosing embryo implantation potential by combining proteomics and time lapse technologies

Wednesday, June 24, 2015
We developed a diagnostic tool using a combination of proteomic fingerprinting and time-lapse morphokinetic analysis to select embryos for transfer according to their implantation potential.

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Predictive value of maternal serum human chorionic gonadotropin levels in pregnancies achieved by in vitro fertilization with single cleavage and single blastocyst embryo transfers

Monday, June 1, 2015
Mean human chorionic gonadotropin levels resulting from a single fresh blastocyst transfer were significantly higher than those from a single cleavage transfer after adjusting for confounding variables.

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Deoxyribonucleic acid methylation profiling of single human blastocysts by methylated CpG island amplification coupled with CpG island microarray

Monday, June 1, 2015
Methylated CpG-island amplification coupled with CpG-island microarray was used to assess DNA methylation in individual in vitro–derived human blastocysts, revealing 121 CpG islands that were hypermethylated in all 5 embryos.

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Frozen thawed day 5 blastocyst transfer is associated with a lower risk of ectopic pregnancy than day 3 transfer and fresh transfer

Friday, February 27, 2015
Frozen-thawed day 5 blastocyst transfer is associated with a lower ectopic pregnancy rate than frozen- thawed day 3 transfer and fresh transfer in patients undergoing IVF-ET.

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In vitro development of donated frozen thawed human embryos in a prototype static microfluidic device A randomized controlled trial

Friday, February 27, 2015
This study shows that a microfluidic device tested under static conditions supports single-embryo culture of donated frozen-thawed human day-4 embryos to advanced blastocyst stages.

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Birth weight is associated with inner cell mass grade of blastocysts

Friday, January 30, 2015

Capsule:
Blastocysts with a more developed inner cell mass developed into newborns with a greater weight, when corrected for gestational age and gender.…

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Blastocyst transfer is not associated with increased rates of monozygotic twins when controlling for embryo cohort quality

Monday, December 29, 2014
The association between blastocyst transfer and monozygotic twinning may be related to the high quality nature of the embryo cohort rather than the extended culture.

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Blastocysts can be rebiopsied for preimplantation genetic diagnosis and screening

Tuesday, November 25, 2014
We found that blastocysts can survive two rounds of biopsy, vitrification, and warming with a high implantation rate. Blastocyst re-examination may be clinically valuable in preimplantation genetic diagnosis/screening cycles.

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Blastocentesis A source of DNA for preimplantation genetic testing Results from a pilot study

Tuesday, November 25, 2014
DNA retrieved by blastocentesis provided a chromosome condition that was highly correspondent with that found in trophectoderm cells. Results from polar bodies and blastomeres were also predictive of the blastocyst’s ploidy.

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Role of assisted hatching in in vitro fertilization A guideline

Wednesday, July 30, 2014
This guideline document reviews the safety and efficacy of assisted hatching.

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Effect of oocytes vitrification on deoxyribonucleic acid methylation of H19 Peg3 and Snrpn differentially methylated regions in mouse blastocysts

Monday, June 23, 2014
Vitrification can lead to a loss of deoxyribonucleic acid methylation of imprinted genes in blastocysts, possibly because of reduction of deoxyribonucleic acid methyltransferase abundance in mouse MII oocytes after vitrification.

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MicroRNAs and implantation

Sunday, June 1, 2014
This review discusses diagnostic and therapeutic roles of miRNA in implantation. Although promising, further research is needed before miRNA are used in clinical practice for identifying and treating implantation failure.

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Single embryo transfer of vitrified warmed blastocysts yields equivalent live birth rates and improved neonatal outcomes compared with fresh transfers

Wednesday, April 30, 2014
After 1,157 fresh and 645 vitrified-warmed day-5 single-blastocyst transfers, we found vitrified warmed blastocysts yielded equivalent birth rates and improved neonatal outcomes compared with fresh transfers.

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Next generation sequencing The dawn of a new era for preimplantation genetic diagnostics

Wednesday, April 30, 2014
Reflections on "Development and validation of a next-generation sequencing–based protocol for 24-chromosome aneuploidy screening of embryos" by Fiorentino et al.

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Obstetric and perinatal outcomes in singleton pregnancies resulting from the transfer of blastocyst stage versus cleavage stage embryos generated through in vitro fertilization treatment A systematic review and meta analysis

Wednesday, November 27, 2013
Singleton pregnancies arising from the transfer of embryos at blastocyst-stage in IVF appear to have higher risk of preterm and very preterm delivery when compared with cleavage-stage transfer.

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Should embryos developing to blastocysts on day 7 be cryopreserved and transferred an analysis of pregnancy and implantation rates

Monday, September 30, 2013
Embryos cryopreserved after reaching blastocyst stage on day 7 were compared to those cryopreserved on days 5 and 6. Day 7 blastocysts have lower but clinically important pregnancy rates.

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Influence of oocyte dysmorphisms on blastocyst formation and quality

Friday, August 30, 2013
Individual identification of oocyte dymorphisms may be a prognostic tool for blastocyst development and quality.

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Comprehensive chromosome screening of trophectoderm with vitrification facilitates elective single embryo transfer for infertile women with advanced maternal age

Friday, August 30, 2013
Comprehensive chromosome screening of trophectoderm with vitrification and later transfer in an unstimulated cycle may be the key to providing elective single-embryo transfer for the older woman undergoing IVF.

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Glycoprotein 130 promotes human blastocyst development in vitro

Tuesday, April 30, 2013
Supplementation of glycoprotein 130 to culture media increased blastocyst formation of human pre-embryos.

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Number of supernumerary vitrified blastocysts is positively correlated with implantation and live birth in single blastocyst embryo transfers

Tuesday, April 30, 2013
The number of supernumerary vitrified blastocysts correlates positively with the odds of implantation and live birth in single-blastocyst transfers.

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Cleavage stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not A randomized and paired clinical trial

Tuesday, April 9, 2013
A paired randomized trial demonstrated a 39% relative reduction in implantation rates of embryos after cleavage-stage biopsy. In contrast, blastocyst biopsy had no adverse effect.

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