Preimplantation genetic screening using fluorescence in situ hybridization improves live-birth rates in patients with repetitive implantation failure or advanced maternal age.
Reflections on “Preimplantation genetic screening using fluorescence in situ hybridization in patients with repetitive implantation failure and advanced maternal age: two randomized trials” by Rubio et al.
The use of array comparative genomic hybridization in preimplantation genetic screening produces high pregnancy rates with embryo biopsy at different stages.
We describe the role of mitochondrial dysfunction in ovarian senescence and suggest a treatment to improve reproductive outcome with mitochondrial nutrients.
Telomere dynamics differ between the female and male germ lines: oocytes have short telomeres, and their erosion recapitulates the reproductive aging phenotype, and telomere length in sperm increases with age.
In this study analyzing embryo morphology and chromosomal status in relation to rate of embryonic developmental progression, the ability to blastulate, rather than day of blastulation, was found to significantly affect aneuploidy.
Author:
Ana Cobo, Ph.D.
Volume 98, Issue 3, Pages 600-601, September 2012
Abstract:
Reflections on “Oocyte vitrification does not increase the risk of embryonic aneuploidy or diminish the implantation potential of blastocysts created after intracytoplasmic sperm injection: a novel, paired randomized controlled trial using DNA fingerprinting”…
Current preimplantation screening results with array comparative genomic hybridization indicatea significant decrease in the miscarriage rate of idiopathic recurrent pregnancy loss patients. Furthermore, this confirms that idiopathic recurrent miscarriage is mostly caused by chromosomal abnormalities in embryos.
Using a novel, paired study design, this RCT demonstrates that blastocysts derived from previously vitrified oocytes are not at an increased risk of aneuploidy and have equivalent reproductive potential.
The increased incidence of aberrant meiosis in in vitro–matured human oocytes correlates with persistence of lysine-9 acetylation of histone-3.
Characterization of ploidy errors in 274 blastomeres using a novel informatics-based technique revealed aneuploidy profiles with unprecedented detail, including identifying parental origin of aneuploidy and differentiating mitotic from meiotic errors.
Array comparative genomic hybridization analysis of mosaic samples can help determine the detectable level of aneuploidy. Aneuploidy could be confidently called when the level of aneuploid cells in the sample was >50%.
High intra-age, intercycle, and intercenter variation in oocyte aneuploidy was observed in first polar body chromosome analysis of oocyctes.
This prospective, blinded, nonselection study is the first to determine the negative and positive predictive values of aneuploidy screening for clinical outcome. Results demonstrate that this CCS method can be safely used to discard embryos because they rarely possess significant reproductive potential.
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