Fertilization and embryo development with spermatozoa obtained from testicular sperm extraction into oocytes generated from human chorionic gonadotropin primed in vitro maturation cycles
The fertilization rate and embryo development of IVM oocytes after intracytoplasmic sperm injection (ICSI) using testicular spermatozoa was lower than that of sibling in vivo-matured oocytes.
Weon-Young Son, Ph.D., Jin-Tae Chung, M.S., Sara Henderson, B.S., Shauna Reinblatt, M.D., William Buckett, M.D., Peter T.K. Chan, M.D., Hananel Holzer, M.D.
Volume 100, Issue 4, Pages 989-993, October 2013
To evaluate the fertilization rate and embryo development resulting from intracytoplasmic sperm injection (ICSI) of spermatozoa retrieved by testicular sperm extraction (TESE) in hCG-primed in vitro maturation (IVM) cycles.
University teaching hospital.
Twenty-four IVM cycles were performed in 21 patients (mean age, 32.3 ± 2.4 years) with polycystic ovaries (PCO) whose partners were nonobstructive azoospermic. Twelve cycles where IVM oocytes were also retrieved were compared with a control group consisting of age-matched IVM cycles with ICSI using ejaculated spermatozoa (n = 12).
In vitro maturation treatment with TESE sperm.
Main Outcome Measure(s):
Fertilization and embryo development between sibling oocytes matured in vivo and in vitro.
Eight singleton pregnancies and one twin pregnancy were obtained after ET (9/24, 37.5%). In the 12 IVM cycles where in vivo-matured oocytes were also obtained, the fertilization rate after TESE-ICSI was significantly higher in in vivo-matured oocytes than in sibling in vitro-matured oocytes (84.2% vs. 53.2%). The proportion of good quality embryos was also higher (63.5% vs. 40.2%). In the control group of cycles with ejaculated spermatozoa, there was no difference in fertilization rates between sibling oocytes matured in vivo and in vitro (84.6% vs. 79.6%).
Our results suggest that IVM of immature oocytes combined with TESE-ICSI is an option for couples with PCO and azoospermia. However, there are lower fertilization and good quality embryo rates achieved when TESE-ICSI was done with in vitro-matured oocytes. Additional studies are necessary to determine the role of this treatment combination.