Human chorionic gonadotropin stimulates spheroid attachment on fallopian tube epithelial cells through the mitogen activated protein kinase pathway and down regulation of olfactomedin 1

Capsule:
Human chorionic gonadotropin increases the attachment of trophoblastic spheroids on human fallopian tube epithelial cells, through activation of extracellular signal regulated kinase and Wnt/b-catenin signaling pathways, leading to down-regulation of Olfm1 expression.

Authors:
Kam-Hei So, M.Phil., Suranga P. Kodithuwakku, Ph.D., Kottawattage S.A. Kottawatta, M.Phil., Raymond H.W. Li, M.B.B.S., Philip C.N. Chiu, Ph.D., Annie N.Y. Cheung, M.D., Ernest H.Y. Ng, M.D., William S.B. Yeung, Ph.D., Kai-Fai Lee, Ph.D.

Volume 104, Issue 2, Pages 474-482

Abstract:

Objective:
To study the effect of human chorionic gonadotropin (hCG) on olfactomedin-1 (Olfm1) expression and spheroid attachment in human fallopian tube epithelial cells in vitro.

Design:
Experimental study.

Setting:
Reproductive biology laboratory.

Patient(s):
Healthy nonpregnant women.

Intervention(s):
No patient interventions.

Main Outcome Measure(s):
Luteinizing hormone/chorionic gonadotropin receptor (LHCGR) and Olfm1 expression in fallopian tube epithelium cell line (OE-E6/E7 cells). OE-E6/E7 cells treated with hCG, U0126 extracellular signal-regulated kinase (ERK) inhibitor, or XAV939 Wnt/β-catenin inhibitor were analyzed by Western blotting, real-time polymerase chain reaction, and in vitro spheroid attachment assay.

Result(s):
Human chorionic gonadotropin increased spheroid attachment on OE-E6/E7 cells through down-regulation of Olfm1 and activation of Wnt and mitogen-activated protein kinase (MAPK) signaling pathways. U0126 down-regulated both MAPK and Wnt/β-catenin signaling pathways and up-regulated Olfm1 expression. XAV939 down-regulated only the Wnt/β-catenin signaling pathway but up-regulated Olfm1 expression.

Conclusion(s):
Human chorionic gonadotropin activated both ERK and Wnt/β-catenin signaling pathways and enhanced spheroid attachment on fallopian tube epithelial cells through down-regulation of Olfm1 expression.

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