Role for the endometrial epithelial protein MFG E8 and its receptor integrin αvβ3 in human implantation Results of an in vitro trophoblast attachment study using established human cell lines

Capsule:
MFG-E8 and its receptor integrin avb3 are involved in the attachment of trophoblasts to the endometrial epithelium.

Authors:
Carla Schmitz, M.D., M.Sc., Slivina Bocca, M.D., Ph.D., Sandra Anderson, B.S., Hai Wang, Ph.D., João Sabino Cunha-Filho, M.D., Ph.D., Bhaskara S. Ravi, Ph.D., Sergio Oehninger, M.D., Ph.D.

Volume 101, Issue 3, Pages 874-882, March 2014

Abstract:

Objective:
To investigate the role of MFG-E8 and its receptor integrin αvβ3 in the attachment of trophoblast cells to the endometrial epithelium.

Design:
Experimental in vitro study.

Setting:
Academic center.

Patient(s):
None.

Intervention(s):
By using a well-differentiated endometrial adenocarcinoma cell line (Ishikawa cells) and choriocarcinoma human trophoblast cells (Jar cells), an in vitro assay mimicking human implantation was established. To investigate the impact of blocking MFG-E8 and integrin αvβ3, we pretreated the cell lines with antibodies against those proteins at different concentrations before the attachment assay.

Main Outcome Measure(s):
Attachment rate of Jar spheroids to the epithelial cell monolayer.

Result(s):
Pretreatment of Ishikawa cells with anti-MFG-E8 antibody caused a dose-dependent and significant inhibition of attachment. On the other hand, pretreatment of Jar spheroids did not result in a significant effect on the attachment rate. Pretreatment of Ishikawa cells as well as Jar spheroids with anti-integrin αvβ3 antibodies resulted in a dose-dependent, significant inhibition of attachment.

Conclusion(s):
This study showed that blocking MFG-E8 and its receptor integrin αvβ3 in Ishikawa cells diminishes Jar spheroid attachment. Moreover, blocking integrin αvβ3 in the trophoblastic cells also diminished their attachment to the Ishikawa monolayer.

Translate »