Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in PVP-based sperm immobilization medium
Polyvinylpyrrolidone-based medium can induce sperm DNA fragmentation during intracytoplasmic sperm injection, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.
Sujit Raj Salian, B.Sc., Guruprasad Kalthur, Ph.D., Shubhashree Uppangala, M.Sc., Pratap Kumar, M.D., Satish Kumar Adiga, Ph.D.
Volume 98, Issue 2, Pages 321-325, August 2012
To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions.
Experimental prospective study.
Embryology research laboratory.
Forty-six ejaculates from normozoospermic and oligozoospermic men.
Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay.
Main Outcome Measure(s):
DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media.
Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control.
A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.