Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in PVP-based sperm immobilization medium

Capsule:
Polyvinylpyrrolidone-based medium can induce sperm DNA fragmentation during intracytoplasmic sperm injection, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

Authors:
Sujit Raj Salian, B.Sc., Guruprasad Kalthur, Ph.D., Shubhashree Uppangala, M.Sc., Pratap Kumar, M.D., Satish Kumar Adiga, Ph.D.

Volume 98, Issue 2, Pages 321-325, August 2012

Abstract:

Objective:
To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNA integrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions.

Design:
Experimental prospective study.

Setting:
Embryology research laboratory.

Patient(s):
Forty-six ejaculates from normozoospermic and oligozoospermic men.

Intervention(s):
Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay.

Main Outcome Measure(s):
DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media.

Result(s):
Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control.

Conclusion(s):
A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.

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