Removal of annexin V positive sperm cells for intracytoplasmic sperm injection in ovum donation cycles does not improve reproductive outcome A controlled and randomized trial in unselected males

Capsule:
Magnetic activated cell sorting of sperm cells from unselected male patients does not increase the live birth delivery rate after intracytoplasmic sperm injection in ovum donation cycles.

Authors:
Laura Romany, Ph.D., Nicolas Garrido, Ph.D., Yamileth Motato, Ph.D., Belen Aparicio, Ph.D., Jose Remohi, M.D., Marcos Meseguer, Ph.D.

Volume 102, Issue 6, Pages 1567-1575

Abstract:

Objective:
To determine the effect of removing presumptive apoptotic sperm cells from samples from unselected males by means of magnetic activated cell sorting (MACS) on live-birth delivery rates after intracytoplasmic sperm injection (ICSI) in couples undergoing ovum donation (OD).

Design:
Prospective, randomized, triple-blinded, and controlled study.

Setting:
Private university-affiliated IVF center.

Patient(s):
A total of 237 infertile couples undergoing ICSI as part of an OD program.

Intervention(s):
Semen specimens from the control group were prepared by swim-up. Samples from the study group were prepared by swim-up followed by MACS and incubation with annexin V–conjugated microbeads to remove annexin V–positive (AV+) sperm cells.

Main Outcome Measure(s):
Fertilization rates, morphological features of early embryo development, implantation rates, ongoing pregnancy rates, and live-birth rates.

Result(s):
Similar results were obtained between groups for all the parameters compared: fertilization rates of 75.3% (95% confidence interval [CI], 71.6–78.9) versus 72.1% (95% CI, 68.6–75.7); percentage of good-quality embryos on day 2 of 53.7% (95% CI, 50.3–57.1) versus 51.8% (95% CI, 48.3–55.3) and on day 3 of 54.2% (95% CI, 50.7–57.6) versus 48.9% (95% CI, 45.3–52.4); implantation rates of 42.2% (95% CI, 33.8–48.1) versus 40.1% (95% CI, 34.8–49.6); positive beta-hCG tests of 63.2% (95% CI, 54.7–71.6) versus 68.6% (95% CI, 60.2–76.9), and live-birth rates of 48.4% (95% CI, 39.6–57.1) versus 56.4% (95% CI, 47.3–65.5) in the MACS versus control group. None of the differences reached statistical significance.

Conclusion(s):
Applying MACS technology to remove AV+ sperm cells from unselected males does not improve the reproductive outcome of ICSI in OD.

Translate »