The expression of estrogen receptors as well as GREB1, c-MYC, and cyclin D1 estrogen regulated genes implicated in proliferation is increased in peritoneal endometriosis

Capsule:
The expression of E receptors as well as GREB1, Cyclin D1, and c-myc is increased in peritoneal endometriotic lesions compared with control eutopic endometrium.

Authors:
Chiara Pellegrini, M.Sc., Ilaria Gori, Ph.D., Chahin Achtari, M.D., Daniela Hornung, M.D., Ph.D., Eric Chardonnens, M.D., Dorothea Wunder, M.D., Maryse Fiche, M.D., and Geraldine O. Canny, Ph.D.

Volume 98, Issue 5, Pages 1200-1208, November 2012

Abstract:

Objective:
To analyze the expression of estrogen receptor α and β as well as their target genes implicated in proliferation, c-myc, Cyclin D1 and GREB1 in the endometrium of women with or without endometriosis.

Design:
Expression analysis in human tissue.

Setting:
University Hospital and clinic.

Patients:
91 premenopausal women (59 endometriosis patients and 32 controls) undergoing laparoscopic surgery.

Intervention:
Biopsies were obtained at time of surgery, performed during the proliferative phase of the cycle.

Main Outcome Measure(s):
Estrogen receptors α and β as well as c-myc, Cyclin D1 and GREB1 mRNA expression levels were quantified by qRT-PCR. Tissue localization of these estrogen-regulated genes was analyzed by immunohistochemistry.

Results:
Estrogen receptors α and β as well as c-myc, Cyclin D1 and GREB1 mRNA expression levels were increased in ectopic in comparison to both normal and eutopic endometrium. Estrogen receptor mRNA levels were also upregulated in eutopic peritoneal tissue of endometriosis patients. Cyclin D1 and GREB1 expression was augmented in eutopic endometrium. c-myc, Cyclin D1 and GREB1 proteins exhibited a nuclear localization in ectopic endometrial tissue.

Conclusions:
This constitutes the first report of increased expression of GREB1, as well as cyclin D1 and c-myc, in peritoneal endometriotic lesions, implicating these proteins in estrogen-dependent growth in this context.

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