Cytokine gene polymorphisms in northern Indian women with recurrent miscarriages

Capsule:
This study highlights the risk involving various genotypes and alleles of the interleukin (IL) 4 (C590T), IL-6 (G174C), IL-10 (1082A/G and 819C/T), and interferon-g (+874 A/T) polymorphism with recurrent miscarriage.

Authors:
Farah Parveen, M.Sc., Anju Shukla, M.D, Suraksha Agrawal, M.Sc., Ph.D.

Volume 99, Issue 2, Pages 433-440.e2, February 2013

Abstract:

Objective:
To determine association of cytokine gene polymorphism with risk for recurrent miscarriages (RM).

Design:
Retrospective case-control study on northern Indian RM cases versus control subjects.

Setting:
Medical facility.

Patient(s):
A total of 200 women with at least three unexplained spontaneous abortions before 20 weeks of gestation.

Intervention(s):
Subjects were genotyped by the PCR amplification followed by restriction digestion based method and allele-specific oligonucleotides method.

Main Outcome Measure(s):
Detection of pro- and antiinflammatory gene polymorphism genotypes and allele frequencies.

Result(s):
We applied dominant and recessive models of inheritance showing no association among Th2: IL-10(592 C/A) and TGF-β gene polymorphisms, while significant association was observed between Th2: IL-4(C590T), IL-6(G174C), IL-10 (1082A/G & 819 C/T) and Th1: IFN-γ(+874 A/T) with RM when compared to controls. However, when CART (classification and regression tree analysis) was applied this effect disappeared and demonstrated that IL-10 plays an important role in maintenance of pregnancy.

Conclusion(s):
IL-10 act as immunosuppressive by keeping a balance of pro and anti-inflammatory signs that coordinate the satisfactory development of pregnancy, placental growth and remodeling, for favorable pregnancy outcome.

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