Effect of antiangiogenic treatment on peritoneal endometriosis associated nerve fibers

Capsule:
Because nerve fibers present in endometriosis play an important role in pain mechanisms, antiangiogenic treatment that decreases nerve fibers provides a novel therapeutic approach.

Authors:
Edurne Novella-Maestre, Ph.D., Sonia Herraiz, Ph.D., José María Vila-Vives, M.D., Carmen Carda, Ph.D., M.D., Amparo Ruiz-Sauri, Ph.D., M.D., Antonio Pellicer, Ph.D., M.D.

Volume 98, Issue 5, Pages 1209-1217, November 2012

Abstract:

Objective:
To investigate the antiangiogenic treatment effect on experimental endometriotic lesion nerve fibers.

Design:
Heterologous mice model of endometriosis.

Setting:
University Institute IVI, University Hospital La Fe.

Animal(s):
Ovariectomized nude mice (n = 16) receiving human endometrial fragments from oocyte donors (n = 4).

Intervention(s):
Endometrium fragments were stuck in the peritoneum of 5-week old female nude mice. Mice were treated with vehicle (n=8) and anti-angiogenic agent Cabergoline (n=8; Cb2, 0.05 mg/kg/day) for 14 days.

Main outcome measure(s):
Immunofluorescence analysis of von-Willebrand factor (vWF) and vascular smooth muscle cells (αSMA) for evaluating the number of immature blood vessels (IBV) and microvascular density (MVD); immunochemical analysis of protein-gene product 9.5 (PGP 9.5) to assess nerve fibers density (NFD), and blue toluidine staining to confirm presence of mast cells and macrophages in endometriotic lesions.

Result(s):
All the results were quantified by morphometric techniques. The IBV, NFD, and number of macrophages and mast cells were statistically significantly decreased in the Cb2-treated group when compared with controls.

Conclusion(s):
Antiangiogenic treatment statistically significantly diminishes new blood vessel formation after macrophage, mast cell, and nerve fiber reduction, providing a rationale to test antiangiogenic agents as a novel therapeutic approach to severe pelvic pain associated with human peritoneal endometriosis.

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