Drospirenone induces decidualization in human eutopic endometrial stromal cells and reduces DNA synthesis of human endometriotic stromal cells

Capsule:
Drospirenone induces decidualization in human eutopic endometrial stromal cells and reduces DNA synthesis of human endometriotic stromal cells.

Authors:
Mariko Miyashita, M.D., Kaori Koga, M.D., Ph.D., Gentaro Izumi, M.D., Ph.D., Tomoko Makabe, M.D., Akiko Hasegawa, M.D., Ph.D., Yasushi Hirota, M.D., Ph.D., Tetsuya Hirata, M.D., Ph.D., Miyuki Harada, M.D., Ph.D., Tomoyuki Fujii, M.D., Ph.D., Yutaka Osuga, M.D., Ph.D.

Volume 104, Issue 1, Pages 217–224

Abstract:

Objective:
To investigate the in vitro effect of drospirenone on human eutopic endometrial (EuSC) and ectopic endometriotic stromal cells (EcSC).

Design:
Comparative and laboratory study. The experimental procedures were approved by the Institutional Review Board of the University of Tokyo (registration no. 0324-4).

Setting:
University research laboratory.

Patients(s):
Eight patients undergoing hysterectomy for benign gynecologic disease and 19 patients undergoing cystectomy or adnectomy for endometriosis.

Intervention(s):
EuSC and EcSC were treated with drospirenone.

Main Outcome Measure(s):
For the analysis of decidualization of EuSC, cells were observed using microscopy, and the production of PRL was measured using enzyme immunoassay. For the analysis of DNA synthesis of EcSC, 5-bromo-2’-deoxyuridine incorporation was measured by ELISA. Cells in apoptosis were detected and measured by flow cytometry.

Result(s):
Drospirenone induced decidualization in EuSC, and the induction was negated by RU486. Drospirenone reduced DNA synthesis on EcSC, and this reduction was negated by RU486 or P receptor silencing, but not by aldosterone or mineralocorticoid receptor silencing. Drospirenone did not cause EcSC to undergo apoptosis.

Conclusion(s):
Our study demonstrates the direct effects of drospirenone: decidualization of EuSC and reduced DNA synthesis of EcSC, but it does not cause EsSC apoptosis.

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