The Effect of Timing of Embryonic Progression on Chromosomal Abnormality

Capsule:
In this study analyzing embryo morphology and chromosomal status in relation to rate of embryonic developmental progression, the ability to blastulate, rather than day of blastulation, was found to significantly affect aneuploidy.

Authors:
Lindsay Kroener, M.D., Gayane Ambartsumyan, M.D., Ph.D., Christine Briton-Jones, Ph.D., Daniel Dumesic, M.D., Mark Surrey, M.D., David Hill, Ph.D., H.C.L.D.

Volume 98, Issue 4, Pages 876-880, October 2012

Abstract:

Objective:
To evaluate the relationship between aneuploidy and timing of blastocyst formation.

Design:
Historical cohort study.

Setting:
Private IVF clinic.

Patient(s):
Ninety-four couples undergoing IVF treatment in combination with chromosomal screening of embryos. The mean maternal age was 39.2 years and average number of embryos per patient was 5.3.

Intervention(s):
A total of 530 embryos were biopsied on day 3 and underwent chromosome screening with microarray-based comparative genomic hybridization.

Main Outcome Measure(s):
Effect of day of embryo blastulation and morphologic grade on aneuploidy rate.

Result(s):
Day 5 morulas that progressed to blastocysts on day 6 were significantly less likely to be aneuploid (79.8%) than day 5 morulas that did not progress to blastocysts (92.9%). However, there was no significant difference in aneuploidy rates when embryos that became blastocysts on day 5 were directly compared with embryos that became blastocysts on day 6.

Conclusion(s):
Delayed blastulation is not associated with increased aneuploidy rates, but absence of blastulation is associated with increased aneuploidy. Therefore, we conclude that when choosing a morula for transfer on day 5, there may be a benefit in waiting an extra day for the possibility of blastulation to occur.

  • Paul Brezina

    This article confirms much of the current thinking surrounding aneuploidy in early embryogenesis. Furthermore, this article offers significant clinical applications; specifically that embryos that reach the blastocyst stage (either on day 5 or 6) are equally as likely to possess euploid embryos.

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