Aging sperm negatively impacts in vivo and in vitro reproduction a longitudinal murine study

Capsule:
Natural male aging negatively affects both in vivo and in vitro reproductive outcomes in an outbred murine model.

Authors:
Mandy G. Katz-Jaffe, Ph.D., Jason Parks, B.Sc., Blair McCallie, B.Sc., William B. Schoolcraft, M.D.

Volume 100, Issue 1, Pages 262-268.e2, July 2013

Abstract:

Objective:
To investigate the impact of paternal aging on reproductive success.

Design:
Animal study.

Setting:
Research facility.

Animal(s):
Outbred CF1 mice.

Intervention(s):
Ten young male mice with proven fertility were mated routinely over 15 months with superovulated young females to assess in vivo and in vitro reproductive outcome.

Main Outcome Measure(s):
In vivo fertilization, in vivo fetal development, in vitro embryo morphology, and developmental outcome were assessed.

Result(s):
There were no differences observed for any reproductive end point until the paternal age of 12 months. At 12–15 months, in vivo fertilization was significantly decreased (35% vs. 78% at <12 months). Natural matings with males ≥12 months revealed significantly smaller fetuses (11.36 mm vs. 14.73 mm <12 months) and placental weight (0.10 g vs. 0.13 g at <12 months). In vitro blastocyst development showed a significant decline at ≥12 months, and in vitro blastocyst transfer resulted in a significant increase in pregnancy loss with males ≥12 months (61.5% vs. 0% at <12 months). In addition, the expression levels of Ace-1, Prm1, Prm2, and Smcp were observed to be decreased in sperm from males ≥12 months compared with young male control subjects. Conclusion(s):
Results from this study indicate an abrupt reproductive deterioration at paternal midlife, with an adverse effect observed on natural conception, in vitro blastocyst development, implantation potential, and fetal viability.

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