Investigation of gene expression profiles before and after embryonic genome activation and assessment of functional pathways at the human metaphase II oocyte and blastocyst stage
Gene expression profiling at the human oocyte and blastocyst stage has identified similarities and differences in processes before and after embryonic genome activation.
Georgia Kakourou, Ph.D., Souraya Jaroudi, Ph.D., Pinar Tulay, M.Sc., Carleen Heath, M.Sc., Paul Serhal, M.D., Joyce C. Harper, Ph.D., Sioban B. SenGupta, Ph.D.
Volume 99, Issue 3, Pages 803-814.e23, 1 March 2013
Objective: To compare the oocyte versus the blastocyst transcriptome and provide data on molecular pathways pre- and post- embryonic genome activation.
Prospective laboratory research study.
An IVF clinic and a specialist preimplantation genetics laboratory.
Couples undergoing or having completed IVF treatment donating surplus oocytes or cryopreserved blastocysts following patient consent.
Sets of pooled metaphase II oocytes or blastocysts were processed for RNA extraction, RNA amplification and analysis using the Human Genome Survey Microarrays V2.0 (Applied Biosystems).
Main Outcome Measure(s):
Association of cell type and gene expression profile.
Totals of 1,909 and 3,122 genes were uniquely expressed in human MII oocytes and human blastocysts respectively, and 4910 genes were differentially expressed between the two sample types. Expression levels of 560 housekeeping genes, genes involved in the microRNA processing pathway, as well as hormones and hormone receptors were also investigated.
The lists of genes identified may be of use for understanding the processes involved in early embryo development, blastocyst implantation, and identifying any dysregulation leading to infertility.