Relationship between sperm aneuploidy sperm DNA integrity chromatin packaging traditional semen parameters and recurrent pregnancy loss

Capsule:
The increase in abnormal sperm parameters, sperm DNA fragmentation, chromatin decondensation, and aneuploidy suggest possible causes of unexplained recurrent pregnancy loss, which encourage the screening of both partners simultaneously.

Authors:
Ines Zidi-Jrah, M.D., Amani Hajlaoui, M.Sc., Soumaya Mougou-Zrelli, M.D., Ph.D., Molka Kammoun, M.D., Imene Meniaoui, M.D., Amira Sallem, M.D., Sonia Brahem, Ph.D., Meriem Fekih, M.D., Mohammed Bibi, M.D., Ali Saad, M.D., Ph.D., Samira Ibala-Romdhane, M.D., Ph.D.

Volume 105, Issue 1, Pages 58-64

Abstract:

Objective:
To study the possible relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss (RPL).

Design:
Descriptive study.

Setting:
University-affiliated tertiary teaching hospital, cytogenetic and reproductive biology department.

Patient(s):
A total of 22 couples with history of RPL and 20 fertile men.

Intervention(s):
Semen samples from case and control men were examined for differences in semen parameters, DNA fragmentation, chromatin condensation, and sperm aneuploidy.

Main Outcome Measure(s):
Sperm DNA and chromatin integrity and sperm aneuploidy.

Result(s):
Sperm progressive motility (30.2% vs. 51.5%) was significantly lower and abnormal morphology (74.8% vs. 54.2%) was significantly higher in the RPL group versus the control group, respectively. The percentage of fragmented DNA was significantly increased in the RPL group (17.1% vs. 10.2%) as well as the rate of spermatozoa with nuclear chromatin decondensation (23.6% vs. 11.8%). There was a significantly higher sperm aneuploidy rate among the RPL group as well.

Conclusion(s):
The increase in abnormal sperm parameters, sperm DNA fragmentation, nuclear chromatin decondensation, and sperm aneuploidy suggest possible causes of unexplained RPL.

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