Relationship between sperm aneuploidy sperm DNA integrity chromatin packaging traditional semen parameters and recurrent pregnancy loss

Capsule:
The increase in abnormal sperm parameters, sperm DNA fragmentation, chromatin decondensation, and aneuploidy suggest possible causes of unexplained recurrent pregnancy loss, which encourage the screening of both partners simultaneously.

Authors:
Ines Zidi-Jrah, M.D., Amani Hajlaoui, M.Sc., Soumaya Mougou-Zrelli, M.D., Ph.D., Molka Kammoun, M.D., Imene Meniaoui, M.D., Amira Sallem, M.D., Sonia Brahem, Ph.D., Meriem Fekih, M.D., Mohammed Bibi, M.D., Ali Saad, M.D., Ph.D., Samira Ibala-Romdhane, M.D., Ph.D.

Volume 105, Issue 1, Pages 58-64

Abstract:

Objective:
To study the possible relationship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional semen parameters, and recurrent pregnancy loss (RPL).

Design:
Descriptive study.

Setting:
University-affiliated tertiary teaching hospital, cytogenetic and reproductive biology department.

Patient(s):
A total of 22 couples with history of RPL and 20 fertile men.

Intervention(s):
Semen samples from case and control men were examined for differences in semen parameters, DNA fragmentation, chromatin condensation, and sperm aneuploidy.

Main Outcome Measure(s):
Sperm DNA and chromatin integrity and sperm aneuploidy.

Result(s):
Sperm progressive motility (30.2% vs. 51.5%) was significantly lower and abnormal morphology (74.8% vs. 54.2%) was significantly higher in the RPL group versus the control group, respectively. The percentage of fragmented DNA was significantly increased in the RPL group (17.1% vs. 10.2%) as well as the rate of spermatozoa with nuclear chromatin decondensation (23.6% vs. 11.8%). There was a significantly higher sperm aneuploidy rate among the RPL group as well.

Conclusion(s):
The increase in abnormal sperm parameters, sperm DNA fragmentation, nuclear chromatin decondensation, and sperm aneuploidy suggest possible causes of unexplained RPL.

  • msamplaski

    The authors provide increasing data for the role of sperm DNA health in RPL. This will likely help to, at the very least, give couples more answers. I would be interested in knowing what defined “fertile” how many of these men were on antioxidant supplements, as well as lifestyle modifications (smoking and MJ) and had varicoceles. Finally, what would be most helpful would be to take these same couples who had RPL using ejaculated sperm, and do IVF with TESE sperm and see if there was a decline in RPL rates.

  • Ranjith Ramasamy

    The authors present more evidence that sperm aneuploidy and sperm DFI / decondensation can explain causes of RPL. This study further validates findings from our study (Ramasamy et al. F&S 2015) and the study from Cornell (Mehta et al. F&S 2015) for men with recurrent pregnancy loss and recurrent IVF failure. Tests of sperm chromatin structure (Sperm aneuploidy, sperm DFI) can finally have a clear indication (RPL and recurrent IVF failure).

  • Jason M. Franasiak

    An interesting study detailing differences in male reproductive parameters in the RPL population. Given the sperm methylation data discussed in this issue, was there any differences in environmental factors (male BMI, male smoking status, etc.) which might be linked to poorer sperm parameters and performance?

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