Detection and quantification of maternal effect gene transcripts in mouse second polar bodies potential markers of embryo developmental competence
Differences in gene expression in individual second polar bodies may be potential markers for minimally invasive testing of embryo developmental competence.
Ze-Xu Jiao, M.D., Ph.D., Teresa K. Woodruff, Ph.D.
Volume 99, Issue 7, Pages 2055-2061, June 2013
To test the hypothesis that quantification of messenger RNAs originating from the second polar body (PB2) provides a noninvasive tool for assessing embryo quality.
Hospital-based academic research laboratory.
CD1 female mice.
Metaphase II oocytes obtained from 7- to 8-week-old mice after pregnant mare’s serum gonadotropin and hCG priming. After in vitro fertilization, the PB2 was biopsied from zygote, followed by reverse transcription. Real-time polymerase chain reaction was performed to quantify gene expression levels in single PB2. The sibling zygotes were continuously cultured to blastocyst stage.
Main Outcome Measure(s):
Embryo developmental competence and six maternal-effect gene (Dnmt1, Mater, Nobox, Npm2, Tcl1, and Zar1) transcripts in the PB2.
Second polar body messenger RNA was detected in all candidate genes. Transcripts that were present in greater abundance in the zygote were more likely to be detected in quantitative polymerase chain reaction replicates from single PB2. Four candidate genes (Dnmt1, Nobox, Npm2, and Tcl1) expression levels in PB2 between two groups (two-cell embryo vs. blastocyts) approached statistical significance.
Second polar bodies may contain a representative transcript profile to that of the zygote after fertilization. Differences in gene expression in PB2 may be potential biomarkers of embryo quality.