Endometriosis may be associated with mitochondrial dysfunction in cumulus cells from subjects undergoing in vitro fertilization intracytoplasmic sperm injection as reflected by decreased adenosine triphosphate production

Capsule:
In subjects with surgically confirmed endometriosis, cumulus cells obtained after IVF stimulation and oocyte retrieval have decreased mitochondrial activity compared with controls.

Authors:
Albert L. Hsu, M.D., Paige M. Townsend, M.Ph., Sergio Oehninger, M.D., Ph.D., Frank J. Castora, Ph.D.

Volume 103, Issue 2, Pages 347-352

Abstract:

Objective:
To determine whether endometriosis is associated with mitochondrial dysfunction in cumulus (granulosa [GC]) cells of subjects undergoing IVF-intracytoplasmic sperm injection (ICSI).

Design:
Prospective cohort study.

Setting:
An IVF clinic in a tertiary academic care center.

Patient(s):
Eleven women with endometriosis and 39 controls.

Intervention(s):
None.

Main Outcome Measure(s):
Cumulus cell adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA), and genomic DNA (gDNA) number.

Result(s):
Cumulus cell ATP content was 65% lower in subjects with surgically proven endometriosis (median 312.5 attomoles/ng total DNA, interquartile range = 116.0–667.8) compared with controls (median 892.4 attomoles/ng total DNA, interquartile range = 403.0–1,412.2). There was no significant difference in mtDNA:gDNA ratio. There were no significant differences in age, body mass index (BMI), basal serum FSH level, total oocyte number, metaphase II (M2) oocyte number, metaphase I oocyte number, percentage of M2 oocytes, fertilization rate, implantation rate, or pregnancy rate (PR). Multivariate regression analysis showed significant positive correlations between ATP and [1] M2 oocyte number (r = 0.307) and [2] pregnancy (r = 0.332). There were also trends toward positive correlations between ATP and [3] age (r = 0.283), [4] total number of oocytes (r = 0.271), [5] percentage of M2 oocytes (r = 0.249), and [6] implantation rate (r = 0.293). There were no statistically significant correlations between mtDNA:gDNA ratio and any demographic factors or clinical outcomes measured.

Conclusion(s):
Surgically confirmed endometriosis may be associated with cumulus cell mitochondrial dysfunction in subjects undergoing IVF-ICSI for infertility, as reflected by decreased ATP production.

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