Prenatal hyperandrogenism and lipid profile during different age stages An experimental study

Data presented here reveal the importance of evaluating the complete lipid profile, especially at early stages of life after the prenatal hyperandrogenism condition.

Florencia Heber, Silvana Ferreira, Leandro Velez, Alicia B. Motta, Ph.D.

Volume 99, Issue 2, Pages 551-557, February 2013


The present study investigates the effect of prenatal hyperandrogenization on lipid metabolism and oxidant/antioxidant balance.

Experimental study.

Research institute.

Pregnant Sprague Dawley rats were subcutaneously injected with 2mg free testosterone between days 16 and 19 of pregnancy while controls (C) with vehicle (0.1 ml sesame oil). Prenatally hyperandrogenized female offspring (T2) resemble a polycystic ovary condition. Animals were weighed and killed at 21 and 60 days of age (N=15 rats/group).

Ovarian tissue and truncal blood of control (C) and prenatally hyperandrogenized rats (T2) were obtained.

Main Outcome Measure(s):
Circulating lipid profile (total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol and triglycerides) was quantified by colorimetric-enzymatic methods. Ovarian oxidative stress was evaluated by quantifying lipid peroxidation and glutathione content by spectofotometric assays. Ovarian fat content was evaluated by Red Oil Staining and ovarian messenger RNA (mRNA) expression of peroxisome proliferator-activated receptor gamma (PPARγ) by Real Time polymerase chain reaction (Real Time-PCR).

At 60 days of age, the 100 % of rats from C and the 20 % of rats from T2 ovulated. At 21 days of age T2 rats displayed lower body weight (BW) than C; however, at 60 days of age T2 and C showed similar BW. The lipid profile (total cholesterol, LDLcholesterol, HDL-cholesterol and triglycerides) was altered in both, the anovulatory and ovulatory phenotype of T2 group, but they are higher in the anovulatory phenotype. Lipid peroxidation of rats at 21 and 60 days of age from T2 was similar to C but the antioxidant glutathione was decreased in 21-day-old rats as compared to C. The lipid content of ovarian tissue, determined by Red Oil Staining, was higher in T2 than C group. The mRNA expression of ovarian PPARγ, quantified by Real Time PCR, decreased in anovulatory rats at 60 days of age from T2 as compared to C rats.

Our findings reveal the importance of evaluating the complete lipid profile, especially at early stages of life after the prenatal hyperandrogenism condition. In addition, we novelty demonstrated that the antioxidant GSH would represent a good marker of oxidative stress since it is altered before lipid peroxidation. Prenatal hyperandrogenization also alters the gene expression of PPARγ in rats. Here we demonstrated for the first time that abnormalities in PPARγ and lipid profile were higher in rats showing an anovulatory phenotype than those displaying an ovulatory phenotype.

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