Interferometric phase microscopy for label free morphological evaluation of sperm cells

Capsule:
Sperm imaging is typically performed using label-based bright-field microscopy, which cannot be used for intracytoplasmic sperm injection. Interferometric phase microscopy was used to evaluate sperm cell morphology without labeling.

Authors:
Miki Haifler, M.D., Pinhas Girshovitz, M.Sc., Gili Band, Ph.D., Gili Dardikman, Igal Madjar, M.D., Natan T. Shaked, Ph.D.

Volume 104, Issue 1, Pages 43–47

Abstract:

Objective:
To compare label-free interferometric phase microscopy (IPM) to label-free and label-based bright-field microscopy (BFM) in evaluating sperm cell morphology. This comparison helps in evaluating the potential of IPM for clinical sperm analysis without staining.

Design:
Comparison of imaging modalities.

Setting:
University laboratory.

Patient(s):
Sperm samples were obtained from healthy sperm donors.

Intervention(s):
We evaluated 350 sperm cells, using portable IPM and BFM, according to World Health Organization (WHO) criteria. The parameters evaluated were length and width of the sperm head and midpiece; size and width of the acrosome; head, midpiece, and tail configuration; and general normality of the cell.

Main Outcome Measure(s):
Continuous variables were compared using the Student’s t test. Categorical variables were compared with the χ2 test of independence. Sensitivity and specificity of IPM and label-free BFM were calculated and compared with label-based BFM.

Result(s):
No statistical differences were found between IPM and label-based BFM in the WHO criteria. In contrast, IPM measurements of head and midpiece width and acrosome area were different from those of label-free BFM. Sensitivity and specificity of IPM were higher than those of label-free BFM for the WHO criteria.

Conclusion(s):
Label-free IPM can identify sperm cell abnormalities, with an excellent correlation with label-based BFM, and with higher accuracy compared with label-free BFM. Further prospective clinical trials are required to enable IPM as part of clinical sperm selection procedures.

Translate »