The disturbance of TH17 Treg cell balance in adenomyosis

Capsule:
The disturbance of TH17-Treg cell balance was involved in the immunopathogenesis of both diffuse and focal forms of adenomyosis and showed positive correlation with dysmenorrhea severity and CA-125 levels.

Authors:
Tao Gui, M.D., Chen Chen, M.D., Zhenzhen Zhang, M.D., Weiwei Tang, M.D., Ruyun Qian, M.D., Xiaoping Ma, M.D., Peng Cao, Ph.D., Guiping Wan, Ph.D.

Volume 101, Issue 2, Pages 506-514, February 2014

Abstract:

Objective:
To investigate the balance between regulatory T cells (Treg) and T-helper 17 cells (TH17) in peripheral blood and uteri of women with adenomyosis (AM), and to evaluate their potential correlation with dysmenorrhea and CA-125 levels.

Design:
Laboratory study using human peripheral blood and tissues.

Setting:
Academic hospital.

Patient(s):
Forty-five patients with AM (study group) and 25 women without AM (control group).

Intervention(s):
The peripheral blood and tissues harvested from all groups were subjected to flow cytometry, ELISA, quantitative real-time polymerase chain reaction, and immunohistochemistry. The severity of dysmenorrhea was distinguished by visual analog scale (VAS).

Main Outcome Measure(s):
TH17 and Treg cell frequency, mRNA and protein levels of transcription factors and cytokines in all groups, and their correlation between the TH17-Treg ratio and dysmenorrhea severity or CA-125 level.

Result(s):
The disturbance of TH17-Treg balance was demonstrated in peripheral circulation and uteri of patients with both diffuse and focal AM, and it correlated positively with dysmenorrhea severity and CA-125.

Conclusion(s):
The findings suggest that TH17-Treg imbalance may play a crucial role in the immunopathogenesis of AM, and may be thus a potential target of AM therapy.

Clinical Trial Registration Number:
ChiCTR-CCC-13003500.

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