Expression of microtubule associated protein 2 and synaptophysin in endometrium High levels in deep infiltrating endometriosis lesions
Nerve growth factor, microtubule associated protein 2, and synaptophysin messenger RNAs and proteins are expressed in healthy endometrium and in endometriosis, being highest in deep lesions.
Martina Gori, B.Sc., Alice Luddi, Ph.D., Giuseppe Belmonte, B.Sc., Paola Piomboni, Ph.D., Claudia Tosti, M.D., Lucia Funghi, Ph.D., Errico Zupi, M.D., Lucia Lazzeri, M.D., Ph.D., Felice Petraglia, M.D.
Volume 105, Issue 2, Pages 435-443
To assess whether healthy endometrium, eutopic endometrium, and endometriotic lesions express nerve growth factor (NGF), microtubule-associated protein 2 (MAP-2), and synaptophysin (SYP).
Molecular study in tissue extracts.
A group of women (n = 70), divided as  healthy controls (n = 30) and  with endometriosis (n = 40), was included.
From the healthy control group an endometrial specimen was collected by hysteroscopy (proliferative phase, n = 16; secretive phase, n = 14). Endometriotic and endometrial specimens were collected from women undergoing laparoscopic surgery for endometriosis, endometrioma (OMA) (n = 20), or deep infiltrating endometriosis (DIE) (n = 20).
Main Outcome Measure(s):
To assess expression of NGF, MAP-2, and SYP messenger RNA (mRNA) levels in endometrium and in endometriosis by real-time polymerase chain reaction and protein localization by immunofluorescence. Cultures of human endometrial stromal cells were used to evaluate the effect of tumor necrosis factor (TNF)-α on NGF and SYP.
Endometrial tissue from control expressed mRNA for NGF, MAP-2, and SYP, without any difference between proliferative and secretive phase. The DIE and OMA lesions showed the highest NGF mRNA expression, significantly higher than in eutopic endometrium and control. In DIE lesions SYP mRNA expression was higher than in OMA or in eutopic endometrium or controls. Immunofluorescence analysis of NGF, MAP-2, and SYP showed a slightly more intense positive signal in endometriotic lesions. Exposure to TNF-α increased NGF and SYP mRNA expression in endometrial culture cells.
The present study revealed the presence of two selected neuronal markers, MAP-2 and SYP mRNAs and protein expression, in eutopic endometrium and in endometriotic lesions.