Blastocentesis A source of DNA for preimplantation genetic testing Results from a pilot study

DNA retrieved by blastocentesis provided a chromosome condition that was highly correspondent with that found in trophectoderm cells. Results from polar bodies and blastomeres were also predictive of the blastocyst’s ploidy.

Luca Gianaroli, M.D., M. Cristina Magli, M.Sc., A. Pomante, Ph.D., Anna M. Crivello, B.Sc., G. Cafueri, B.Sc., M. Valerio, B.Sc., Anna P. Ferraretti, M.D.

Volume 102, Issue 6, Pages 1692-1699


To investigate the presence of DNA in blastocyst fluids (BFs) and to estimate whether the chromosomal status predicted by its analysis corresponds with the ploidy condition in trophectoderm (TE) cells, the whole embryo, and that predicted by polar bodies (PBs) or blastomeres.

Prospective study.

In vitro fertilization unit.

Seventeen couples undergoing preimplantation genetic screening with the use of array comparative genomic hybridization on PBs (n = 12) or blastomeres (n = 5).

BFs and TE cells were retrieved from 51 blastocysts for separate chromosomal analysis.

Main Outcome Measure(s):
Presence of DNA in BFs and assessment of the corresponding chromosome condition; correlation with the results in TE cells and those predicted by the analysis done at earlier stages.

DNA was detected in 39 BFs (76.5%). In 38 of 39 cases (97.4%) the ploidy condition of BFs was confirmed in TE cells, and the rate of concordance per single chromosome was 96.6% (904/936). In relation to the whole embryo, the ploidy condition corresponded in all cases with a per-chromosome concordance of 98.1%. The testing of PBs and blastomeres had 93.3% and 100% prediction of BF ploidy condition with a concordance per chromosome of 93.5% and 94%, respectively.

Blastocentesis could represent an alternative source of material for chromosomal testing, because the BF is highly predictive of the embryo ploidy condition and chromosome content. Our data confirm the relevance of the oocyte and of the early-cleavage embryo in determining the ploidy condition of the resulting blastocyst.

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