Dynamic alterations in the expression and localization of ACTL7a during capacitation in mouse spermatozoa

Capsule:
The expression of ACTL7a is up-regulated via the protein kinase A pathway and undergoes remodeling during the early period of capacitation in mouse spermatozoa.

Authors:
Jun Fu, Ph.D., Wei Song, Ph.D., Shudong Zong, Samuel S. Koide, Ph.D., M.D., Shiying Miao, Linfang Wang

Volume 99, Issue 3, Pages 882-888, 1 March 2013

Abstract:

Objective:
To demonstrate that capacitation in mouse spermatozoa involves alterations in the expression and localization of ACTL7a.

Design:
Determine the expression level and localization of ACTL7a in mouse spermatozoa before and after capacitation.

Setting:
The Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, People’s Republic of China.

Animal(s):
ICR mice.

Intervention(s):
None.

Main Outcome Measurements:
Western Blot, indirect immunostaining.

Results:
The expression of ACTL7a is up-regulated via the PKA pathway and undergoes remodeling during the early period of capacitation in mouse spermatozoa.

Conclusion:
ACTL7a is an essential component of capacitation in mouse spermatozoa. The alteration in the expression and localization of ACTL7a may be the primary biochemical event in the induction of capacitation in mouse spermatozoa.

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