Successful delivery derived from vitrified warmed spermatozoa from a patient with nonobstructive azoospermia

Capsule:
One healthy boy was born after transfer of a blastocyst derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia. The spermatozoa were vitrified with the use of Cryotop and Cell Sleeper.

Authors:
Yuji Endo, B.Sc., Yoshitaka Fujii, Ph.D., Shozo Kurotsuchi, M.D., Ph.D., Hiroaki Motoyama, M.D., Hiroaki Funahashi, Ph.D.

Volume 98, Issue 6, Pages 1423-1427, December 2012

Abstract:

Objective:
To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility.

Design:
Retrospective case series.

Setting:
IVF unit of a medical center.

Patient(s):
Three patients with severe oligozoospermia or nonobstructive azoospermia (NOA).

Intervention(s):
Cryopreservation of limited numbers of spermatozoa using Cryotop and Cell Sleeper as nonbiologic containers.

Main Outcome Measure(s):
Four cycles underwent intracytoplasmic sperm injection (ICSI) with vitrified sperm.

Result(s):
A total of 148 spermatozoa in 18 containers (8.2 sperm per container) were vitrified and 36 of them (5 containers) were warmed. Thirty-three sperm (92%) were retrieved successfully and injected individually into 17 mature oocytes. Fertilization was observed in 12 (71%) oocytes and all zygotes (100%) cleaved. A couple with NOA achieved a singleton pregnancy and concluded with full-term delivery of a healthy boy (2632 g).

Conclusion(s):
A successful delivery was achieved after transfer of a blastocyst derived from vitrified-warmed spermatozoa. A small number of vitrified sperm cells were used for ICSI to fertilize oocytes with predictable timing.

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