Induction of endometriotic nodules in an experimental baboon model mimicking human deep nodular lesions

Capsule:
In the first experimental model of deep nodular endometriosis, lesions similar to endometriotic nodules were induced, and they invaded surrounding organs in more than 40% of cases.

Authors:
Olivier Donnez, M.D., Anne Van Langendonckt, Ph.D., Sylvie Defrère, Ph.D., Sébastien Colette, Ph.D., Olivier Van Kerk, Jean-Paul Dehoux, D.V.M., Ph.D., Jean Squifflet, M.D., Ph.D., Jacques Donnez, M.D., Ph.D.

Volume 99, Issue 3, Pages 783-789.e3, 1 March 2013

Abstract:

Objective:
To establish an experimental model for the study of deep nodular endometriosis.

Design:
Induction of nodular endometriosis in baboons by grafting different uterine specimens to the peritoneal cavity.

Setting:
Institute of Primate Research, Nairobi, Kenya, and Catholic University of Louvain, Brussels, Belgium.

Animals:
Ten baboons were used to develop a model of induced deep nodular endometriosis.

Intervention:
Biopsies of endometrium, endometrium plus the JZ, full uterine thickness and myometrium were grafted to the peritoneum.

Main outcome measure(s):
Macroscopic descriptions were recorded for observed induced lesions. Staining with hematoxylin-eosin was performed for histological evaluation and specific antibodies (CK22, CD10) for immunohistochemical studies. Surface area and volume of lesions, glandular density and surrounding organs invasion were also analyzed.

Results:
The incidence of induced nodular endometriosis was 100%, but the extent depended on the tissue grafted. Lesions induced after grafting specimens containing the JZ were significantly (p<0.05) larger than those not containing the JZ. Surrounding organ invasion was reported in more than 40% of lesions after grafting specimens containing the JZ. Conclusion:
Here we describe the first experimental model of nodular endometriosis. This new model now allows investigation of deeper nodular lesions, as well as invasion phenomena associated with nodular lesions.

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