Effect of vitrification on human oocytes A metabolic profiling study

Capsule:
Global metabolomic and amino acid profiles of spent culture media samples from embryos derived from either fresh or vitrified oocytes showed no statistically significant differences.

Authors:
Francisco Dominguez, Ph.D., Damià Castello, Ph.D., José Remohí, M.D., Carlos Simón, M.D., Ana Cobo, Ph.D.

Volume 99, Issue 2, Pages 565-572.e3, February 2013

Abstract:

Objective:
To evaluate the effect of oocyte vitrification in the metabolomic profile of embryos developed from vitrified and fresh oocytes in our ovum donation program.

Design:
Analysis of the metabolic profiles of spent culture medium samples corresponding to embryos coming from vitrified and fresh oocytes.

Setting:
In vitro fertilization (IVF) unit/metabolomic facility.

Patients:
Oocyte donors between the ages of 18 and 35 years.

Interventions:
Metabolomic profile liquid chromatography coupled with mass spectrometry (LC-MS) of spent media samples.

Main Outcome:
Identification of spent media components and metabolites present and absent coming from vitrified and fresh day-3 embryos.

Results:
We obtained a total of 190 spent media samples: vitrification group (65), fresh group (59), and a matched control media group (66). Multivariate data analysis was performed after global metabolomic and amino acid profiles did not reveal any statistically significant differences in day-3 embryos derived from fresh and vitrified oocytes, indicating that other metabolic differences between the samples (e.g., patient-to-patient variability, analytical variation) are greater than those between the vitrified and fresh sample groups. Univariate statistical analysis revealed a series of possible biomarkers, such as tryptophan, phenylalanine, and 2,5,7,8-tetramethyl-2-(2′-carboxyethyl)-6-hydroxychroman (alpha-CEHC), although only alpha-CEHC was statistically significant after correction for multiple testing.

Conclusions:
Multivariate data analysis did not reveal significant differences between the analysed groups, suggesting that oocyte vitrification does not disturb the embryonic metabolomic profiles.

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