Toward a better follow up of ovarian recovery in young women after chemotherapy with a hypersensitive antimullerian hormone assay
The hypersensitive PicoAMH assay, by its capacity to detect low levels of blood AMH, allows a more accurate prediction of menstrual cycle recovery in young women after chemotherapy.
Christine Decanter, M.D., Maeliss Peigne, M.D., Audrey Mailliez, M.D., Franck Morchhauser, M.D., Audrey Dassonneville, Dewailly Didier, M.D., Pascal Pigny, M.D.
Volume 102, Issue 2, Pages 483–487
To evaluate the utility of a hypersensitive assay for measuring low antimüllerian hormone (AMH) levels in young cancer patients during the ovarian recovery phase of their chemotherapy.
Academic medical center.
Fifty-eight samples drawn at least 3 months after the end of chemotherapy in 30 women having either breast cancer (n = 13) or hematologic malignancies (n = 17) were selected to constitute two equally size groups: amenorrhea (n = 30 samples) or spontaneous cycle (n = 28 samples).
Main Outcome Measure(s):
Serum AMH levels were measured by a conventional AMH ELISA (EIA AMH/MIS) and a hypersensitive ELISA (PicoAMH, AnshLabs) on the same sample.
Using a conventional assay, serum AMH was detectable (≥3 pmol/L) in 6.7% and in 10.7% of the samples corresponding to amenorrheic or cycling patients, respectively (nonsignificant). By contrast, with PicoAMH, serum AMH was detectable (≥0.07 pmol/L) in 71.4% of the samples from cycling women vs. 16.7% of the samples from amenorrheic patients. Multivariate regression analysis showed that among putative contributors, only the menstrual status (r = 0.307) and serum FSH level (r = −0.546) were independently correlated to a detectable serum AMH with the picoAMH assay exclusively.
The picoAMH assay, allowing measurement of very low AMH concentrations in human serum, should refine postchemotherapy ovarian follow-up in young women.