Spermatozoa micro ribonucleic acid 34c level is correlated with intracytoplasmic sperm injection outcomes

Capsule:
Spermatozoa miR-34c levels are correlated with the quality of day-3 embryos, pregnancy rate, implantation rate, and live birth rate and can be used as an indicator of ICSI outcomes.

Authors:
Long Cui, M.Sc., Li Fang, M.Sc., Biwei Shi, M.Sc., Sunquan Qiu, M.Sc., Yinghui Ye, M.D., Ph.D.

Volume 104, Issue 2, Pages 312-317

Abstract:

Objective:
To assess the effects of micro ribonucleic acid (miR)-34b/c expression levels in human spermatozoa on intracytoplasmic sperm injection (ICSI) outcomes.

Design:
Retrospective observational study.

Setting:
In vitro fertilization center.

Patient(s):
A total of 162 patients with idiopathic male infertility who had undergone first ICSI cycles.

Intervention(s):
None.

Main Outcome Measure(s):
The levels of miR-34b/c in spermatozoa were measured using real-time polymerase chain reaction. Fertilization, early cleavage, day-3 good-quality embryo, pregnancy, implantation, and live birth rate were assessed. A receiver operating characteristic curve was employed to analyze the cutoff values.

Result(s):
No correlation was found between the spermatozoa miR-34b/c levels and the 2 pronuclei early cleavage rate. A correlation was seen between an increased level of miR-34c and a higher percentage of good-quality embryos on day 3. Although miR-34b and miR-34c levels were higher in the pregnancy group, compared with the nonpregnancy group, receiver operating characteristic curve analysis showed that miR-34c levels in spermatozoa were more strongly correlated with ICSI treatment outcomes, compared with miR-34b (area under the curve = 0.75). Patients in the miR-34c–positive group were more likely to exhibit higher rates of good-quality embryos, implantation, pregnancy, and live birth. A multivariable logistic regression analysis showed that miR-34c in spermatozoa (odds ratio: 5.699, with 95% confidence interval [CI]: 2.687–12.088) and woman’s age (odds ratio: 0.843, with 95% CI: 0.736–0.966) were the 2 parameters that were significantly correlated with pregnancy.

Conclusion(s):
Our results demonstrate that miR-34c levels in spermatozoa are correlated with ICSI outcomes, suggesting that paternal miR-34c may play a role in the early phases of embryonic development. Levels of MiR-34c in human spermatozoa may be used as an indicator for ICSI outcomes.

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