Use of suboptimal sperm increases the risk of aneuploidy of the sex chromosomes in preimplantation blastocyst embryos

Capsule:
The rate of sex chromosome aneuploidy is significantly higher in the trophectoderm of blastocyst embryos derived from severely oligozoospermic males compared with those with normal semen parameters.

Authors:
Alison Coates, B.Sc., John S. Hesla, M.D., Amanda Hurliman, M.D., Breanne Coate, B.S., Elizabeth Holmes, M.S., Rebecca Matthews, Ph.D., Emily L. Mounts, M.S., Kara J. Turner, Ph.D., Alan R. Thornhill, Ph.D., Darren K. Griffin, D.Sc.

Volume 104, Issue 4, Pages 866-872

Abstract:

Objective:
To compare autosomal and sex chromosome aneuploidy rates of embryos derived from sperm with abnormal and normal parameters.

Design:
Retrospective cohort study.

Setting:
Assisted reproduction center.

Patient(s):
Three thousand eight hundred thirty-five embryos generated from 629 couples undergoing IVF.

Intervention(s):
None.

Main Outcome Measure(s):
Incidence of aneuploidy in the trophectoderm of blastocyst embryos derived from standard IVF embryos and intracytoplasmic (ICSI) males with normal and oligozoospermic semen samples, in couples with donor eggs (mean maternal age, 25.0 years) and their own eggs (mean maternal age, 35.4 years).

Result(s):
The rate of sex chromosome aneuploidy was significantly (around threefold) higher in the oligozoospermic group compared with in both control groups (standard vs. ICSI insemination). This applied whether donor (young) or own (older) eggs were used. Significant differences were seen in the oligozoospermic samples for autosomes 1, 2, 11 (own eggs), and 18 (donor eggs) compared with both control groups; however, no significant difference was seen between each of the treatment groups for the overall rate of autosomal aneuploidy. No significant differences were seen between the two control groups (normozoospermic males, standard vs. ICSI insemination) in either of the egg group types for any chromosome pairs.

Conclusion(s):
Severe male factor infertility is associated with a significant increase in the occurrence of sex chromosome abnormalities in blastocyst embryos compared with in embryos derived from normal semen samples. Aneuploidy rates in embryos derived from sperm with normal parameters were not significantly different whether ICSI or standard insemination was used to achieve fertilization. These results highlight severe male factor infertility as a possible referral category for preimplantation comprehensive chromosomal screening.

  • Daniel J. Kaser, MD

    Dear authors,

    Thanks for this important contribution, which succinctly demonstrates a three-fold increased risk of sex chromosome aneuploidy in TE biopsies from embryos derived from ICSI for severe oligospermia compared to ICSI for non-male factor or IVF with normal semen parameters. Interestingly, the observed effect was independent of female age, and was stable when analyzed using both autologous and donor oocytes. Reassuring information for ICSI itself, and also highlights that couples with severe male factor may benefit from CCS.
    Do you happen to have karyotype information on the males with oligospermia that you could share?

    Congratulations on a nice paper!

Translate »