Circulating microRNAs as potential biomarkers for endometriosis

Capsule:
Several miRNAs are differentially expressed in sera of patients with endometriosis compared with controls; a combination of circulating miRNAs during the proliferative phase yielded high diagnostic performance in discriminating endometriosis.

Authors:
SiHyun Cho, M.D., Levent Mutlu, M.D., Olga Grechukhina, M.D., Hugh S. Taylor, M.D.

Volume 103, Issue 5, Pages 1252-1260

Abstract:

Objective:
To evaluate whether microRNAs (miRNAs) associated with endometriosis are detectable in the circulation and could serve as potential noninvasive biomarkers for endometriosis.

Design:
Case-control study.

Setting:
University hospital.

Patient(s):
Twenty-four women with endometriosis and 24 women without the disease (controls).

Intervention(s):
Serum samples collected from women undergoing laparoscopy for endometriosis and other benign gynecologic disease.

Main Outcome Measure(s):
Total RNA extracted from serum and quantitative reverse-transcription polymerase chain reaction to determine levels of miRNA let-7a–f and miR-135a,b.

Result(s):
The levels of circulating let-7b and miR-135a were statistically significantly decreased in women with endometriosis compared with controls, and let-7d and 7f showed a trend toward down-regulation. Let-7b expression strongly correlated with serum CA-125 levels and showed the highest area under the curve of 0.691. When the patients were analyzed according to phase of the menstrual cycle, the expression of let-7b, 7c, 7d, and 7e was statistically significantly lower in the women with endometriosis during the proliferative phase. Using a logistic regression model, we evaluated the diagnostic power of differently expressed miRNAs; the combination of let-7b, let-7d, and let-7f during the proliferative phase yielded the highest area under the curve value of 0.929 in discriminating endometriosis from controls.

Conclusion(s):
Several circulating miRNAs are differentially expressed in the sera of women with endometriosis compared with controls. The combination of serum let-7b, 7d, and 7f levels during the proliferative phase may serve as a diagnostic marker for endometriosis.

Translate »