Growth differential factor 9 inhibits testosterone production in mouse theca interstitial cells

Capsule:
Growth differential factor-9 can inhibit the production of testosterone in mouse theca interstitial cells and suppress corresponding gene expression.

Authors:
Ming-hui Chen, M.D., Tao Li, Ph.D., Chen-hui Ding, Ph.D., Yan-wen Xu, Ph.D., Lu-yan Guo, M.Sc., Can-quan Zhou, M.D.

Volume 100, Issue 5, Pages 1444-1450, November 2013

Abstract:

Objective:
To explore the effect of growth differential factor-9 (GDF-9) alone on cell proliferation, cell viability, steroidogenesis, and hormone-stimulated gene expression in cultured mouse theca interstitial cells.

Design:
Basic research.

Setting:
University hospital.

Animal(s):
Immature 3- to 4-week-old SPF KM mice obtained from the Laboratory Animal Center of Sun Yat-Sen University.

Intervention(s):
Addition of GDF-9 at different dosages to primary culture of mouse theca interstitial cells.

Main Outcome Measure(s):
Cell number, cell viability, progesterone and testosterone levels, and hormone-stimulated gene mRNA abundance.

Result(s):
Growth differential factor-9 mildly increased the number of mouse theca interstitial cells and cell viability in a dose-dependent manner and mildly inhibited the production of progesterone in mouse theca interstitial cells. Administration of GDF-9 at the dosages of 200 ng/mL and 400 ng/mL resulted in a significant decrease in the testosterone level compared with the control group by 60.42% and 68.76%, respectively. Growth differential factor-9 significantly suppressed Lhcgr mRNA by 47.36%, Cyp11a1 mRNA by 62.30%, and Cyp17a1 mRNA by 55.39%, but had only a mild effect on Star gene expression.

Conclusion(s):
Growth differential factor-9 can inhibit the production of testosterone in mouse theca interstitial cells and suppress the corresponding gene expression.

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