Antimüllerian hormone inhibits follicle stimulating hormone induced adenylyl cyclase activation aromatase expression and estradiol production in human granulosa lutein cells

Capsule:
Antimullerian hormone acts through type II AMH receptor to inhibit FSH-induced adenylyl cyclase activation in hGL cells and subsequently suppresses aromatase expression and E2 production.

Authors:
Hsun-Ming Chang, M.D., Christian Klausen, Ph.D., Peter C.K. Leung, Ph.D., F.R.S.C.

Volume 100, Issue 2, Pages 585-592.e1, August 2013

Abstract:

Objective:
To investigate the effects of antimüllerian hormone (AMH) on basal and FSH-induced cytochrome P450 aromatase (aromatase) expression and E2 production in human granulosa-lutein (hGL) cells, and to elucidate the mechanism by which AMH exerts its effects.

Design:
Experimental study.

Setting:
Academic medical center for reproductive science.

Patient(s):
The hGL cells were obtained from consenting patients undergoing IVF treatment.

Intervention(s):
None.

Main Outcome Measure(s):
Primary cultures of hGL cells were used to examine the effects of AMH (10 ng/mL) on basal and FSH (0.2 IU/mL)-stimulated E2 and intracellular cyclic adenosine 3′:5′ monophosphate (cAMP) accumulation, as well as aromatase and FSH receptor expression. Small interfering RNA targeting type II AMH receptor (AMHR2) was used to verify the specificity of the effects.

Result(s):
Treatment with AMH significantly reduced FSH-stimulated aromatase expression and E2 accumulation, whereas it had no measurable effects on basal and/or 8-Br-cAMP-stimulated levels. The FSH receptor messenger RNA and protein levels were not altered in AMH-treated cells. Cotreatment with AMH suppressed FSH-induced increases in intracellular cAMP. Knockdown of AMHR2 reversed the effects of AMH on aromatase expression.

Conclusion(s):
The AMH acts through AMHR2 to inhibit FSH-induced adenylyl cyclase activation, aromatase expression, and E2 production.

Translate »