Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss A prospective study

Capsule:
Evaluation of the effect of sperm deoxyribonucleic acid fragmentation in male partners of couples with unexplained recurrent pregnancy loss compared with fertile men.

Authors:
Gihan M. Bareh, M.D., Ph.D., Ethan Jacoby, B.A., Peter Binkley, B.S., Tien-cheng “Arthur” Chang, Ph.D., Robert S. Schenken, M.D., Randal D. Robinson, M.D.

Volume 105, Issue 2, Pages 329-336

Abstract:

Objective:
To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL).

Design:
Prospective, cohort study.

Setting:
Academic tertiary care center.

Patient(s):
Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility.

Intervention(s):
Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 106 spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis.

Main Outcome Measure(s):
Sperm DNA fragmentation in both groups.

Result(s):
Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7).

Conclusion(s):
Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

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