Methylation analysis of idiopathic recurrent spontaneous miscarriage cases reveals aberrant imprinting at H19 ICR in normozoospermic individuals

Capsule:
Male partners of couples with idiopathic recurrent spontaneous miscarriages with no significant changes in the basic semen parameters reveal significant methylation aberration in spermatozoa at the H19 ICR.

Authors:
Mandar Ankolkar, M.Sc., Anushree Patil, M.D., Himangi Warke, M.D., Vinita Salvi, M.D., Neelam Kedia-Mokashi, M.Sc., Shilpa Pathak, Ph.D., Nafisa Balasinor, Ph.D.

Volume 98, Issue 5, Pages 1186-1192, November 2012

Abstract:

Objective:
To study H19 ICR methylation levels in association with sperm 47 parameters routinely analyzed in idiopathic recurrent spontaneous miscarriage cases.

Design:
Case-Control study.

Setting:
Academic research setting.

Patient(s):
Male partners of couples with a history of idiopathic Recurrent Spontaneous Miscarriage (RSM group) and Male partners of couples with proven fertility (control group).

Intervention(s):
None.

Main Outcome Measure(s):
Paternal age, sperm concentration, motility, chromatin compaction status, morphology, and H19 ICR methylation were assessed in control and idiopathic RSM group participants

Result(s):
Paternal age and basic semen parameters analyzed did not show any significant difference between the two groups; however H19 ICR methylation levels were reduced significantly at (p<0.01) in the idiopathic RSM group compared with the control group. Conclusion(s):
Significant reduction in the H19 ICR methylation without significant difference in the sperm parameters demonstrates aberrant imprinting to be associated with idiopathic RSM.

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