Prospective study into the value of the automated Elecsys antimüllerian hormone assay for the assessment of the ovarian growing follicle pool
An automated antimullerian hormone assay showed good correlations with age and antral follicle count and is a reproducible measure of the growing follicle pool.
Richard A. Anderson, M.D., Ph.D., Ellen Anckaert, M.D., Ph.D., Ernesto Bosch, M.D., Didier Dewailly, M.D., Cheryl E. Dunlop, M.D., Daniel Fehr, M.D., Luciano Nardo, M.D., Johan Smitz, M.D., Ph.D., Kelton Tremellen, M.D., Ph.D., Barbara Denk, Ph.D., Andrea Geistanger, Ph.D., Martin Hund, Ph.D.
Volume 103, Issue 4, Pages 1074-1080
To evaluate a new fully automated assay measuring antimüllerian hormone (AMH; Roche Elecsys) against antral follicle count in women of reproductive age.
Prospective cohort study.
Hospital infertility clinics and academic centers.
Four hundred fifty-one women aged 18 to 44 years, with regular menstrual cycles.
Main Outcome Measure(s):
AMH and antral follicle count (AFC) determined at a single visit on day 2–4 of the menstrual cycle.
There was a statistically significant variance in AFC but not in AMH between centers. Both AFC and AMH varied by age (overall Spearman rho −0.50 for AFC and −0.47 for AMH), but there was also significant between-center variation in the relationship between AFC and age but not for AMH. There was a strong positive correlation between AMH and AFC (overall spearman rho 0.68), which varied from 0.49 to 0.87 between centers. An agreement table using AFC cutoffs of 7 and 15 showed classification agreement in 63.2%, 56.9% and 74.5% of women for low, medium, and high groups, respectively.
The novel fully automated Elecsys AMH assay shows good correlations with age and AFC in women of reproductive age, providing a reproducible measure of the growing follicle pool.