Intra-age, intercenter, and intercycle differences in chromosome abnormalities in oocytes

Capsule:
High intra-age, intercycle, and intercenter variation in oocyte aneuploidy was observed in first polar body chromosome analysis of oocyctes.

Authors:
Santiago Munné, Ph.D., Karsten R. Held, M.D., Cristina M. Magli, Ph.D., Baris Ata, M.D., Dagan Wells, Ph.D., Elpida Fragouli, Ph.D., Vera Baukloh, Ph.D., Robert Fischer, M.D., Luca Gianaroli, M.D.
Volume 97, Issue 4 , Pages 935-942, April 2012

Objective:
To determine the extent of intra-age and intercycle variations in the frequency of first polar body aneuploidy in two consecutive cycles of oocyte retrieval undertaken by the same patient within 1 year.

Design:
Retrospective study.

Setting:
Fertility centers.

Patient(s):
Infertile couples undergoing IVF.

Intervention(s):
Patients underwent two consecutive cycles of preimplantation genetic screening through first polar body biopsy within 1 year.

Main Outcome Measure(s):
Meiosis I aneuploidy.

Result(s):
A total of 226 patients underwent 452 cycles of preimplantation genetic screening. Differences within age groups were wide, with 0–100% of oocytes being chromosomally normal in all age groups. Euploidy rates between centers were significantly different (48% vs. 25%). Intercycle differences for the same patient were also wide (0–100%), but with 68.5% of patients having less than ±2 euploid eggs of difference between cycles.

Conclusion(s):
Although euploidy rate decreased on average with advancing maternal age, the high intra-age and intercenter variation in oocyte chromosome abnormalities emphasize the difficulty in estimating how many euploid oocytes a specific woman will have. This may have repercussions for fertility preservation where a defined number of eggs are currently frozen just based on maternal age.

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