11-deoxy prostaglandin F2α, a thromboxane A2 receptor agonist, partially alleviates embryo crowding in Lpar3(−/−) females

Capsule:
11-deoxy prostaglandin F2a, a thromboxane A2 receptor (TP) agonist, partially alleviates embryo crowding and increases litter size in the Lpar3(/) females.

Authors:
Xiaoqin Ye, M.D., Ph.D., Honglu Diao, Ph.D., Jerold Chun, M.D., Ph.D.
Volume 97, Issue 3 , Pages 757-763, March 2012

Objective:
To determine cyclooxygenase-derived prostanoid signaling in alleviating embryo crowding in the Lpar3(−/−) females.

Design:
Experimental mouse model.

Setting:
Research laboratories.

Animal(s):
Wild-type, Lpar3(+/−), and Lpar3(−/−) mice.

Intervention(s):
Intraperitoneal (IP) administration of prostaglandin E2 (PGE2), cPGI (a stable analogue of PGI2), and 11-deoxy prostaglandin F2α (11-deoxy PGF2α, a thromboxane A2 receptor agonist) to preimplantation gestation day 3.5 Lpar3(−/−) females.

Main Outcome Measure(s):
Implantation sites were detected by blue dye reaction and embryo spacing was determined by the distribution of the implantation sites along the uterine horns on gestation day 4.5; pregnancy outcome was measured by litter size at birth.

Result(s):
Administration of PGE2 + cPGI on gestation day 3.5 Lpar3(−/−) females restored on-time implantation but did not affect embryo spacing or the number of implantation sites detected on gestation day 4.5; PGE2 + cPGI treatment increased litter size at birth. Administration of PGE2 + cPGI + 11-deoxy PGF2α on gestation day 3.5 Lpar3(−/−) females rescued on-time implantation, partially dispersed the clustered implantation sites normally observed in the Lpar3(−/−) females, increased the number of implantation sites detected on gestation day 4.5, and increased litter size at birth.

Conclusion(s):
The thromboxane A2 receptor agonist 11-deoxy PGF2α can partially alleviate embryo crowding in the Lpar3(−/−) females and embryo crowding likely contributes to reduced litter size in the Lpar3(−/−) females.

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