Origins and rates of aneuploidy in human blastomeres

Capsule:
Characterization of ploidy errors in 274 blastomeres using a novel informatics-based technique revealed aneuploidy profiles with unprecedented detail, including identifying parental origin of aneuploidy and differentiating mitotic from meiotic errors.

Authors:
Matthew Rabinowitz, Ph.D., Allison Ryan, Ph.D., George Gemelos, Ph.D., Matthew Hill, Ph.D., Johan Baner, Ph.D., Cengiz Cinnioglu, Ph.D., Milena Banjevic, Ph.D., Dan Potter, M.D., Dmitri A. Petrov, Ph.D., Zachary Demko, Ph.D.
Volume 97, Issue 2 , Pages 395-401, February 2012

Objective:
To characterize chromosomal error types and parental origin of aneuploidy in cleavage-stage embryos using an informatics-based technique that enables the elucidation of aneuploidy-causing mechanisms.

Design:
Analysis of blastomeres biopsied from cleavage-stage embryos for preimplantation genetic screening during IVF.

Setting:
Laboratory.

Patient(s):
Couples undergoing IVF treatment.

Intervention(s):
Two hundred seventy-four blastomeres were subjected to array-based genotyping and informatics-based techniques to characterize chromosomal error types and parental origin of aneuploidy across all 24 chromosomes.

Main Outcome Measure(s):
Chromosomal error types (monosomy vs. trisomy; mitotic vs. meiotic) and parental origin (maternal vs. paternal).

Result(s):
The rate of maternal meiotic trisomy rose significantly with age, whereas other types of trisomy showed no correlation with age. Trisomies were mostly maternal in origin, whereas paternal and maternal monosomies were roughly equal in frequency. No examples of paternal meiotic trisomy were observed. Segmental error rates were found to be independent of maternal age.

Conclusion(s):
All types of aneuploidy that rose with increasing maternal age can be attributed to disjunction errors during meiosis of the oocyte. Chromosome gains were predominantly maternal in origin and occurred during meiosis, whereas chromosome losses were not biased in terms of parental origin of the chromosome. The ability to determine the parental origin for each chromosome, as well as being able to detect whether multiple homologs from a single parent were present, allowed greater insights into the origin of aneuploidy.

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