Selection of high-potential embryos by culture in poly(dimethylsiloxane) microwells and time-lapse imaging

Capsule:
Individual human embryos with the potential to develop to high-scoring blastocysts could be selected at 2–3 days of culture using this system by examining the times required to complete the second and third mitotic divisions.

Authors:
Shu Hashimoto, Ph.D., Nobuhiro Kato, Ph.D., Kazuhiro Saeki, Ph.D., Yoshiharu Morimoto, M.D., Ph.D.
Volume 97, Issue 2 , Pages 332-337, February 2012

Objective:
To assess the developmental kinetics of human embryos and their ability to develop to morphologically normal blastocysts.

Design:
Experimental study on human embryos donated for research using a time-lapse imaging system based on individual embryo culture in poly(dimethylsiloxane) microwells and monitored using a microscope inside the incubator.

Setting:
Private fertility clinic.

Patient(s):
Surplus embryos donated by couples after undergoing fertility treatment.

Intervention(s):
None.

Main Outcome Measure(s):
Blastocyst score and times required from beginning to completion of the second and third mitotic divisions.

Result(s):
The time required for completion of the second division (the three- to four-cell stage) was shorter in embryos that developed to high-scoring blastocysts (0.7 hours, n = 17) than in those forming low-scoring blastocysts (3.7 hours, n = 24). Similarly, the mean time required to completion of the third division (five- to eight-cell stage) was also significantly shorter in embryos forming high-scoring blastocysts (5.7 hours) than among those forming low-scoring blastocysts (16.9 hours).

Conclusion(s):
Individual embryos with the potential to develop to high-scoring blastocysts could be selected at 2–3 days of culture using this system by examining the times required to complete the second and third mitotic divisions.

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